GABAergic signaling in primary lens epithelial and lentoid cells and its involvement in intracellular Ca2+ modulation

• Published in: Cell calcium (Edinburgh) Vol. 50; no. 4; pp. 381 - 392
• Main Authors: Schwirtlich, Marija, Kwakowsky, Andrea, Emri, Zsuzsa, Antal, Károly, Lacza, Zsombor, Cselenyák, Attila, Katarova, Zoya, Szabó, Gábor
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.10.2011
• Subjects: Action Potentials - drug effects; Advanced Basic Science; Animals; Animals, Newborn; Baclofen - pharmacology; Bicuculline - pharmacology; Calcium - metabolism; Calcium Channels - physiology; Calcium Signaling - drug effects; Calcium Signaling - physiology; Cell differentiation; Cell Differentiation - drug effects; Cell Differentiation - physiology; Epithelial Cells - cytology; Epithelial Cells - metabolism; GABA Agonists - pharmacology; GABA Antagonists - pharmacology; GABA receptors; GABA signaling in development; GAD; gamma-Aminobutyric Acid - pharmacology; Intracellular calcium; LEC; Lens; Lens, Crystalline - cytology; Lens, Crystalline - growth & development; Lens, Crystalline - metabolism; Mice; Muscimol - pharmacology; Neurotransmitters; Primary Cell Culture; Receptors, GABA-A - metabolism; Receptors, GABA-B - metabolism; GABA-B receptor; Intracellular calcium; artificial aqueous humor; vesicular GABA transporter; voltage-gated calcium channel(s); glutamic acid decarboxylase, 67 kDa form; VGAT; GABA-B receptor R2 subunit; GABA receptor(s); GABA-A receptor(s); GABA receptors; GABA Aβ3; GABA AR; glutamic acid decarboxylase, 65 kDa form; postnatal day 0; N-cad; AAH; [Ca 2+] i; DAPI; 4′,6-diamidino-2-phenylindole; lens epithelial cell(s); gamma-aminobutyric acid; GAD; P30; central nervous system; GABA signaling in development; GABA BR; postnatal day 30; GABA; Lens; GAT; GABA-A receptor β3 subunit (GABA-binding); intracellular Ca 2+ concentration; CNS; E-cadherin; N-cadherin; E-cad; connexin 43; GABAR; P0; LEC; GAD67; Cell differentiation; GAD65; GABA transporter (membrane); GABA BR2; Neurotransmitters; Cx43; VGCC; GABAAβ3; GABAAR; [Ca2+]i; GABABR2; GABABR
• ISSN: 0143-4160; 1532-1991
• DOI: 10.1016/j.ceca.2011.07.002

Highlights ► We demonstrate functional GABA signaling in primary mouse lens epithelial cell cultures. ► Glutamic acid decarboxylase, GABA receptors, transporters and GABA are all abundantly expressed. ► GABAA and GABAB receptor agonists or GABA induce synergistic rise of intracellular Ca2+ . ► Persistent activation of the GABAB receptors induces wave propagation of the Ca2+ wave and persistent oscillations. ► The GABA receptor mediated Ca2+ responses are sensitive to antagonists and extracellular Ca2+.