Cloning and structural and expressional characterization of BcpLH gene preferentially expressed in folding leaf of Chinese cabbage

• Published in: Science China. Life sciences Vol. 43; no. 3; pp. 321 - 329
• Main Authors: Yu, X, Peng, J, Feng, X, Yang, S, Zheng, Z, Tang, X, Shen, R, Liu, P, He, Y
• Format: Journal Article
• Language: English
• Published: China Springer Nature B.V 01.06.2000; State Key Laboratory of Plant Molecular Genetics,Shanghai Institute of Plant Physiology,Chinese Academy of Sciences,Shanghai 200032,China
• Subjects: Brassica oleracea; Cloning; DNA probes; Double-stranded RNA; Leaves; RNA-binding protein; Rosette; leafy head; auxin; gene expression; Chinese cabbage; RNA binding protein
• ISSN: 1006-9305; 1674-7305; 1862-2798; 1869-1889
• DOI: 10.1007/bf02879292

Vegetative growth of Chinese cabbage undergoes the four successive stages which are characterized with the definite types of juvenile, rosette, folding and head leaves. From shoot tips of Chinese cabbage at early folding stage, we constructed a cDNA library and screened the differentially expressed cDNA clones using the cDNAs derived from developing folding leaves and rosette leaves as probes. One complete length of cDNA clone is designated asBcpLH. Computer alignment matched BcpLH to the domains of double-stranded RNA binding (DBRM) and the homologous regions were recognized between BcpLH and human and mouse double-stranded RNA-binding protein TRBP. PCR expression analysis shows that during vegetative growthBcpLH gene was expressed preferentially in folding leaves at folding stage. Transcripts ofBcpLH gene were increased when plants were sprayed with IAA. It is deduced thatBcpLH gene may be related to initiation of folding leaf and leafy head and induced by auxin in the aspect of transcriptional expression.