LncRNA MEG3 impacts proliferation, invasion, and migration of ovarian cancer cells through regulating PTEN

• Published in: Inflammation research Vol. 67; no. 11-12; pp. 927 - 936
• Main Authors: Wang, Juelan, Xu, Wenqian, He, Yangke, Xia, Qi, Liu, Siwei
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.12.2018; Springer Nature B.V
• Subjects: Allergology; Animals; Apoptosis; Biomedical and Life Sciences; Biomedicine; Biotechnology; Cancer; Cell Cycle; Cell growth; Cell Line, Tumor; Cell migration; Cell Movement; Cell Proliferation; Dermatology; Female; Flow cytometry; Humans; Immunology; Mice, Inbred BALB C; Mice, Nude; Neurology; Original Research Paper; Ovarian cancer; Ovarian Neoplasms - genetics; Ovarian Neoplasms - metabolism; Ovarian Neoplasms - pathology; Pharmacology/Toxicology; PTEN Phosphohydrolase - genetics; PTEN protein; Rheumatology; RNA, Long Noncoding - genetics; Tumor cell lines; Wound healing; Xenografts; Xenotransplantation; LncRNA MEG3; Ovarian cancer; PTEN
• ISSN: 1023-3830; 1420-908X; 1420-908X
• DOI: 10.1007/s00011-018-1186-z

Objective and design We investigated the expressions of lncRNA MEG3 and PTEN in ovarian cancer tissues and their effects on cell proliferation, cycle and apoptosis of ovarian cancer. Methods Expression levels of MEG3 in ovarian cancer cell lines and normal ovarian cell lines were detected by qRT-PCR. Cell viability was detected by MTT assay. Cell apoptosis and cell cycle distribution were measured by flow cytometry. Cell invasion capability was tested by transwell assay. Cell migration capacity was tested by wound healing. The xenograft model was constructed to explore the effect of lncRNA MEG3 on ovarian cancer in vivo. Result Compared with normal ovarian cells, expression levels of MEG3 and PTEN were relatively lower in ovarian cancer cells. There was a positive correlation between the expression of PTEN and the expression of MEG3. Enhanced expression level of PTEN suppressed SKOV3 cell proliferation, increased cell apoptosis rate, and decreased cell invasion and migration. Conclusion LncRNA MEG3 and PTEN were down-regulated in ovarian cancer cells. LncRNA MEG3 regulated the downstream gene PTEN in ovarian cancer cells to prohibit cell proliferation, promote apoptosis and block cell cycle progression.