Genomic DNA binding to ZnO microrods

• Published in: Applied surface science Vol. 347; pp. 131 - 138
• Main Authors: Guzmán-Embús, D.A., Cardozo, M. Orrego, Vargas-Hernández, C.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 30.08.2015
• Subjects: Backbone; Bands; Binding; Bonding; Cellular; Deoxyribonucleic acid; DNA binding; Raman spectroscopy; SERS; Wurtzite; Zinc oxide; ZnO rods; Raman spectroscopy; SERS; DNA binding; ZnO rods
• ISSN: 0169-4332; 1873-5584
• DOI: 10.1016/j.apsusc.2015.04.067

Figure shows a scheme of interaction between ZnO rods with DNA strand and presents a Van der Waals force attraction between positive rod charges, generated by Zn+2 with the phosphodioxy group (OPO−) DNA backbone. The improvement of the DNA Raman signal is due to changes in the charge distribution in the ZnO–DNA complex. Shifts in the bands from both the (OPO−) group and the CO stretching modes in the complex chain C-O-P-O-C indicate compressive and tensile stresses of the bonds. •ZnO microrods were produced by hydrothermal synthesis.•DNA was extracted from pork spleen cells by cellular lysis, deproteinization and precipitation.•The analysis of the DNA binding to the ZnO was performed using Raman spectroscopy a technique that allowed for the evaluation of the effect that the presence of the ZnO in the complex has on the DNA structure.•We show the compression and tensile stresses generated at the ZnO/DNA interface.•The relative vibrational mode intensities of the nitrogenous bases is increased. In this work, ZnO microrods were produced by hydrothermal synthesis. DNA was extracted from pork spleen cells by cellular lysis, deproteinization and precipitation. The analysis of the DNA binding to the ZnO was performed using Raman spectroscopy a technique that allowed for the evaluation of the effect that the presence of the ZnO in the complex has on the DNA structure. Vibrational spectral bands from the DNA molecule and hexagonal wurtzite ZnO were observed and classified as E2(M), A1(TO), E2(High), E1(LO) and 2LO. The Raman signals from the vibrational bands corresponding to the phosphodiester bond 5′-C-O-P-O-C-3′ and bond stretching of the PO2− group, as well as ring vibrations of the nitrogenous bases of the DNA, were enhanced by the presence of the ZnO microrods. The bands from the modes corresponding to the C-O and OPO− molecules of the DNA backbone were observed to exhibit larger spectral shifts due to the compression and tensile stresses generated at the ZnO/DNA interface, respectively. In addition, the relative vibrational mode intensities of the nitrogenous bases increased.