Personalized Approach to Assessing mRNA Expression of Histidine-Rich Glycoprotein and Immunohistochemical Markers in Diseases of the Breast

• Published in: Doklady. Biochemistry and biophysics Vol. 484; no. 1; pp. 59 - 62
• Main Authors: Autenshlyus, A. I., Golovanova, A. V., Studenikina, A. A., Brusentsov, I. I., Proskura, A. V., Zhurakovskiy, I. P., Arkhipov, S. A., Sidorov, S. V., Vavilin, V. A., Lyakhovich, V. V.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.05.2019; Springer Nature B.V
• Subjects: Adolescent; Adult; Aged; Antigens, CD - biosynthesis; Antigens, CD - genetics; Benign; Biochemistry; Biological and Medical Physics; Biomarkers, Tumor - biosynthesis; Biomarkers, Tumor - genetics; Biomedical and Life Sciences; Biophysics; Biopsy; Breast; Breast cancer; Breast Neoplasms - genetics; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Cadherins - biosynthesis; Cadherins - genetics; Collagen (type II); Diseases; E-cadherin; Epithelial-Mesenchymal Transition; Female; Gene expression; Gene Expression Regulation, Neoplastic; Histidine; Humans; Immunohistochemistry; Invasiveness; Life Sciences; Mesenchyme; Middle Aged; Molecular Biology; Neoplasm Proteins - biosynthesis; Neoplasm Proteins - genetics; Proteins - genetics; Proteins - metabolism
• ISSN: 1607-6729; 1608-3091
• DOI: 10.1134/S1607672919010162

Biopsy material of patients with malignant and benign breast diseases was examined. HRG mRNA expression was detected in 70% of cases in biopsy material obtained from patients with nonspecific invasive carcinoma and in 66.7% of cases in biopsy material of patients with benign breast diseases. Immunohistochemical analysis revealed expression of collagen II, the beta-1 integrin, and E-cadherin—markers of epithelial–mesenchymal transition. The use of RT-qPCR combined with immunohistochemical study made it possible to identify atypical cells, which can be regarded as precancerous changes, in individual patients.