Characterization of bacterial populations in Danish raw milk cheeses made with different starter cultures by denaturating gradient gel electrophoresis and pyrosequencing
The bacterial populations in Danish raw milk cheeses were identified using denaturating gradient gel electrophoresis (DGGE) of PCR amplicons of the V3 region of the 16S rRNA gene and pyrosequencing of tagged amplicons of the V3 and V4 regions of the 16S rRNA gene. Both DNA and RNA extracted from che...
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Published in | International dairy journal Vol. 21; no. 3; pp. 142 - 148 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford
Elsevier Ltd
01.03.2011
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | The bacterial populations in Danish raw milk cheeses were identified using denaturating gradient gel electrophoresis (DGGE) of PCR amplicons of the V3 region of the 16S rRNA gene and pyrosequencing of tagged amplicons of the V3 and V4 regions of the 16S rRNA gene. Both DNA and RNA extracted from cheeses were studied in order to determine the metabolically active bacteria. The main bacteria, which included
Lactococcus,
Lactobacillus and
Streptococcus, were detected by pyrosequencing and DGGE in both 16S rDNA and cDNA obtained from cheeses indicating their viability and contribution to cheese ripening. Other bacteria like
Corynebacterium,
Halomonas,
Pediococcus,
Micrococcus and
Staphylococcus, which were encountered in some cheese samples at low percentages compared with the total bacterial populations, were only detected by pyrosequencing. 16S rRNA gene pyrosequencing is an efficient method for deep sequencing of microbial communities and it expands our knowledge of the bacterial diversity in raw milk cheese. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0958-6946 1879-0143 |
DOI: | 10.1016/j.idairyj.2010.10.007 |