Susceptibility of fall armyworm, Spodoptera frugiperda (J.E. Smith), to eight insecticides in China, with special reference to lambda-cyhalothrin

• Published in: Pesticide biochemistry and physiology Vol. 168; p. 104623
• Main Authors: Zhao, Yun-Xia, Huang, Jing-Mei, Ni, Huan, Guo, Di, Yang, Feng-Xia, Wang, Xin, Wu, Shun-Fan, Gao, Cong-Fen
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.09.2020
• Subjects: acetylcholinesterase; amino acids; arthropods; artificial diets; azadirachtin; China; Chlorantraniliprole; chlorfenapyr; corn; genes; indoxacarb; insect pests; Insect resistance management; insecticide resistance; lambda-cyhalothrin; lufenuron; organophosphorus compounds; phosphates; physiology; piperonyl butoxide; resistance management; Spinetoram; Spodoptera frugiperda; synergism; Target-site mutation; toxicity; China; Spodoptera frugiperda; Chlorantraniliprole; Target-site mutation; Spinetoram; Insect resistance management
• ISSN: 0048-3575; 1095-9939; 1095-9939
• DOI: 10.1016/j.pestbp.2020.104623

Fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith), is the main destructive insect pest of grain crops that occurs in all maize growing regions of the Americas. It has rapidly invaded the Southern China since January 2019. However, the current status of insecticide resistance in S. frugiperda has not been reported in China. In this study, we determined the susceptibility of eight populations of FAW to eight insecticides by an artificial diet incorporation method. The results showed that among eight insecticides, emamectin benzoate, spinetoram, chlorantraniliprole, chlorfenapyr, and lufenuron showed higher toxicity to this pest, while lambda-cyhalothrin and azadirachtin exhibited lower toxicity. Susceptibility of S. frugiperda to indoxacarb was significantly different (10.0-fold for LC50) across the various geographic populations. To investigate the biochemical mechanism of FAW to lambda-cyhalothrin, we performed the synergism tests and the results showed that piperonyl butoxide (PBO) and triphenyl phosphate (TPP) produced a high synergism of lambda-cyhalothrin effects in the two field populations. Sequencing of the gene encoding the acetylcholinesterase (AChE) gene in the two field populations identified two amino acid mutations, all of which have been shown previously to confer resistance to organophosphates (OPs) in several arthropod species. The results of this study provided valuable information for choosing alternative insecticides and for insecticide resistance management of S. frugiperda. [Display omitted] •The susceptibility of eight insecticides to fall armyworm (FAW) were investigated.•Cytochrome P450 monooxygenases contribute to lambda-cyhalothrin resistance in FAW.•Two mutations of acetylcholinesterase gene were detected in the field populations.