Effects of Selenium-Enriched Yeast Improved Aflatoxin B1-Induced Changes in Growth Performance, Antioxidation Capacity, IL-2 and IFN-γ Contents, and Gene Expression in Mice

• Published in: Biological trace element research Vol. 191; no. 1; pp. 183 - 188
• Main Authors: Liu, Lina, Chen, Fu, Qin, Shunyi, Ma, Jifei, Li, Liuan, Jin, Tianming, Zhao, Ruili
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.09.2019; Springer Nature B.V
• Subjects: Aflatoxin B1; Aflatoxin B1 - toxicity; Animals; Antioxidants; Biochemistry; Biomedical and Life Sciences; Biotechnology; Body length; Body weight gain; Capacity; Control; Cytochrome; Cytochromes; Diet; Dietary Supplements; Enrichment; Female; Gene expression; Gene Expression Regulation - drug effects; Glutathione; Glutathione peroxidase; Growth; Interferon; Interleukin 2; Life Sciences; Liver; Male; Malondialdehyde; Mice; Nutrition; Oncology; Peroxidase; Selenium; Selenium - pharmacology; Serum; Sodium Selenite - pharmacology; Toxicity; Yeast; Yeast, Dried - pharmacology; Yeasts; γ-Interferon; Interleukin-2; Antioxidation capacity; Interferon-γ; Aflatoxin B1; Mice; Selenium-enriched yeast; Growth performance
• ISSN: 0163-4984; 1559-0720
• DOI: 10.1007/s12011-018-1607-4

Sixty Kunming mice were randomly assigned into three groups. Mice in a control group were fed a basal diet, while mice in AFB1 group and AFB1-Se group were fed the basal diet supplemented with 250 μg/kg AFB1 or the basal diet supplemented with 250 μg/kg AFB1 and 0.2 mg/kg selenium as selenium-enriched yeast, respectively. On day 30 of the experiment, growth performance, glutathione peroxidase (GSH-Px) activities, total antioxidant capacity (T-AOC) levels, and malondialdehyde (MDA) contents in liver, interleukin-2 (IL-2), and interferon-γ (IFN-γ) contents in serum, and cytochrome P3a11 (Cyp3a11), IL-2, IFN-γ, and GSH-Px1 mRNA levels in liver were determined. The results showed that final weights, weight gains, T-AOC levels, GSH-Px1, and IFN-γ mRNA levels in AFB1-Se group and control group were higher or significantly higher than those in AFB1 group ( P  < 0.05 or P  < 0.01), respectively. Body length gains in AFB1 group were lower than those in the control group ( P  < 0.05), while there was no significant difference between the AFB1-Se and control groups ( P  > 0.05). IL-2 contents and liver IL-2 mRNA levels in AFB1-Se group were significantly higher than those in the AFB1 group and control group ( P  < 0.01), and IL-2 contents in the control group were also significantly higher than those in the AFB1 group ( P  < 0.01). IFN-γ contents in AFB1-Se group and AFB1 group were significantly higher than those in control group ( P  < 0.01), while IFN-γ contents in AFB1-Se group were significantly lower than those in AFB1 group ( P  < 0.01). Cyp3a11 mRNA levels in AFB1-Se group and AFB1 group were significantly higher than those in the control group ( P  < 0.01). The results indicated that selenium-enriched yeast could partly reduce the toxicity induced by AFB1 in mice, including improving growth performance, antioxidation capacity, IL-2 and IFN-γ contents, and enhancing IL-2, IFN-γ, and GSH-Px1 mRNA levels.