Immunophenotyping of Rhesus CMV‐Specific CD8 T‐Cell Populations

• Published in: Cytometry. Part A Vol. 99; no. 3; pp. 278 - 288
• Main Authors: Pomplun, Nicholas L., Vosler, Logan, Weisgrau, Kim L., Furlott, Jessica, Weiler, Andrea M., Abdelaal, Hadia M., Evans, David T., Watkins, David I., Matano, Tetsuro, Skinner, Pamela J., Friedrich, Thomas C., Rakasz, Eva G.
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.03.2021; Wiley Subscription Services, Inc
• Subjects: Antibodies; CD8 antigen; CD8 T‐cell epitope; Cytomegalovirus; Cytometry; Epitopes; Flow cytometry; Gene frequency; immunophenotyping; in situ tetramer staining; Lymphocytes; Lymphocytes T; Macaca mulatta; Major histocompatibility complex; Original; Pathogenicity; Pathogens; Phenotypes; Populations; Proteins; Reagents; rhesus cytomegalovirus; Staining; Transplantation; Vaccine development; Vaccines; CD8 T-cell epitope; in situ tetramer staining; immunophenotyping; rhesus cytomegalovirus
• ISSN: 1552-4922; 1552-4930
• DOI: 10.1002/cyto.a.24197

A vaccine to ameliorate cytomegalovirus (CMV)‐related pathogenicity in transplantation patients is considered a top priority. A therapeutic vaccine must include components that elicit both neutralizing antibodies, and highly effective CD8 T‐cell responses. The most important translational model of vaccine development is the captive‐bred rhesus macaque (Macaca mulatta) of Indian origin. There is a dearth of information on rhesus cytomegalovirus (rhCMV)‐specific CD8 T cells due to the absence of well‐defined CD8 T‐cell epitopes presented by classical MHC‐I molecules. In the current study, we defined two CD8 T‐cell epitopes restricted by high‐frequency Mamu alleles: the Mamu‐A1*002:01 restricted VY9 (VTTLGMALY aa291‐299) epitope of protein IE‐1, and the Mamu‐A1*008:01 restricted NP8 (NPTDRPIP aa96‐103) epitope of protein phosphoprotein 65‐2. We developed tetramers and determined the level, phenotype, and functional capability of the two epitope‐specific T‐cell populations in circulation and various tissues. We demonstrated the value of these tetramers for in situ tetramer staining. Here, we first provided critical reagents and established a flow cytometric staining strategy to study rhCMV‐specific T‐cell responses in up to 40% of captive‐bred rhesus macaques. © 2020 The Authors. Cytometry Part A published by Wiley Periodicals LLC on behalf of International Society for Advancement of Cytometry.