Intestinal fatty acid binding protein Ala54Thr polymorphism is associated with peripheral atherosclerosis combined with type 2 diabetes mellitus

• Published in: Journal of diabetes Vol. 9; no. 9; pp. 821 - 826
• Main Authors: Khattab, Salma A., Abo‐Elmatty, Dina M., Ghattas, Maivel H., Mesbah, Noha M., Mehanna, Eman T.
• Format: Journal Article
• Language: English
• Published: Melbourne Wiley Publishing Asia Pty Ltd 01.09.2017; John Wiley & Sons, Inc
• Subjects: 2型糖尿病; Adult; Alanine - genetics; Atherosclerosis; Body mass index; Case-Control Studies; Diabetes; Diabetes Mellitus, Type 2 - complications; Diabetes Mellitus, Type 2 - genetics; Egypt; Enzyme-Linked Immunosorbent Assay; Fatty Acid-Binding Proteins - genetics; Fatty acids; fatty acid‐binding protein (FABP2); Gene Frequency; Genotype & phenotype; Heterozygote; Humans; Low density lipoprotein; Middle Aged; Peripheral Arterial Disease - complications; Peripheral Arterial Disease - genetics; peripheral atherosclerosis; Polymerase Chain Reaction; polymorphism; Polymorphism, Genetic; Polymorphism, Restriction Fragment Length; Threonine - genetics; type 2 diabetes mellitus; 外周动脉粥样硬化; 多态性; 脂肪酸结合蛋白(FABP2); fatty acid-binding protein (FABP2); peripheral atherosclerosis; type 2 diabetes mellitus; polymorphism; 多态性; 脂肪酸结合蛋白(FABP2); 外周动脉粥样硬化; 2型糖尿病
• ISSN: 1753-0393; 1753-0407
• DOI: 10.1111/1753-0407.12496

Background Intestinal fatty acid‐binding protein 2 (FABP2) is expressed in enterocytes and binds saturated and unsaturated long‐chain fatty acids. The FABP2 Ala54Thr polymorphism has been reported to effect lipid metabolism. The aim of the present study was to assess the relationship between this polymorphism and peripheral atherosclerosis combined with type 2 diabetes mellitus (T2DM) in an Egyptian population. Methods The study was performed on 100 T2DM patients with peripheral atherosclerosis and 100 control subjects. The Ala54Thr polymorphism was analyzed by polymerase chain reaction–restriction fragment length polymorphism, whereas serum FABP2 levels were determined using ELISA. Fasting blood glucose, fasting serum insulin concentrations, HbA1c, lipid profile, body mass index (BMI) and systolic and diastolic blood pressure (SBP and DBP, respectively) were determined. Results There was a higher frequency of the Thr54 allele among the patient group (P = 0.002). In Ala54/Thr54 heterozygotes and carriers of the rare Thr54/Thr54 genotype, there were significant increases in BMI and FABP2. Those with the Thr54/Thr54 genotype had significantly decreased high‐density lipoprotein cholesterol (HDL‐C) concentrations; in addition, those with the Thr54/Thr54 genotype had significantly higher SBP and DBP than subjects with the Ala54/Ala54 and Ala54/Thr54 genotypes. There was a positive correlation between FABP2 levels and BMI, SBP and DBP, and a negative correlation with HDL‐C. Conclusions The Thr54 allele of the FABP2 Ala54Thr polymorphism was associated with an increased incidence of peripheral atherosclerosis combined with T2DM in the population studied. 摘要 背景 肠脂肪酸结合蛋白2(Intestinal fatty acid‐binding protein 2,FABP2)是在肠上皮细胞中表达的,它可以结合饱和以及不饱和长链脂肪酸。既往已有研究报告FABP2 Ala54Thr的多态性可以影响脂类代谢。本研究的目的是在一个埃及人群中评估这种多态性与外周动脉粥样硬化合并2型糖尿病(T2DM)之间的关系。 方法 本研究在100名T2DM合并外周动脉粥样硬化的患者以及100名对照的受试者中进行。使用聚合酶链反应‐限制性片段长度多态性分析评估Ala54Thr的多态性,使用ELISA方法测定血清中的FABP2水平。还测定了空腹血糖、空腹血清胰岛素浓度、HbA1c、血脂谱、体重指数(BMI)、收缩压与舒张压(分别测定SBP与DBP)。 结果 在患者组中Thr54等位基因出现的频率更高(P=0.002)。在Ala54/Thr54杂合子以及罕见的Thr54/Thr54基因型携带者中,BMI与FABP2都显著升高。携带Thr54/Thr54基因型的受试者高密度脂蛋白胆固醇(HDL‐C)浓度显著下降;另外,携带Thr54/Thr54基因型的受试者的SBP与DBP都明显高于携带Ala54/Ala54或者Ala54/Thr54基因型的受试者。FABP2水平与BMI、SBP以及DBP正相关,与HDL‐C负相关。 结论 在这项人群研究中FABP2 Thr54等位基因的Ala54Thr多态性与外周动脉粥样硬化合并T2DM的发病率增加相关。 Relationship between Ala54Thr polymorphism genotypes and different biochemical parameters in the patient group and Relationship between Ala54Thr polymorphism genotypes and different biochemical parameters in the control group Highlights The Thr54 allele of the fatty acid‐binding protein 2 (FABP2) Ala45Thr polymorphism was associated with an increased incidence of type 2 diabetes mellitus (T2DM) combined with peripheral atherosclerosis. There was a positive correlation between FABP2 levels and body mass index and systolic and diastolic blood pressure, and a negative correlation with high‐density lipoprotein cholesterol. To the best of our knowledge, the present study is the first to link a genetic variation in the FABP2 gene with peripheral atherosclerosis in T2DM patients.