Rapid determination of memantine in human plasma by using nanoring carboxyl-functionalized paramagnetic molecularly imprinted polymer d-μ-SPE and UFLC-MS/MS

• Published in: Drug testing and analysis Vol. 7; no. 6; p. 535
• Main Authors: Qiu, Hai-Wen, Xia, Lei, Gong, Li-Min, Ruan, Lie-Min, Zhao, Yong-Gang
• Format: Journal Article
• Language: English
• Published: England 01.06.2015
• Subjects: Chromatography, High Pressure Liquid; Cysteine - analogs & derivatives; Cysteine - chemistry; Humans; Magnetite Nanoparticles - chemistry; Male; Memantine - analysis; Memantine - blood; Methacrylates - chemistry; Molecular Imprinting - methods; Solid Phase Extraction - methods; Tandem Mass Spectrometry; ultra-fast liquid chromatography-tandem quadrupole mass spectrometry (UFLC-MS/MS); dispersive micro-solid-phase extraction (d-μ-SPE); nanoring carboxyl-functionalized paramagnetic molecularly imprinted polymer (NR-CF-Mag-MIP); human plasma; memantine (ME)
• ISSN: 1942-7611
• DOI: 10.1002/dta.1706

A novel, simple, and sensitive method based on the use of dispersive micro-solid-phase extraction (d-μ-SPE) procedure combined with ultra-fast liquid chromatography-tandem quadrupole mass spectrometry (UFLC-MS/MS) for the determination of memantine (ME) was developed and validated over the linearity range 0.05-10.0 µg/L with 100 μL of human plasma using memantine-D6 (ME-D6) as the internal standard. The novel nanoring carboxyl-functionalized paramagnetic molecularly imprinted polymer (NR-CF-Mag-MIP) was synthesized by ultrasound-assisted suspension polymerization, using ME as a template molecule, methacrylic acid as a functional monomer, and divinylbenzene as a cross-linking agent. The NR-CF-Mag-MIP was used as the d-μ-SPE sorbent to extract ME from human plasma samples. The obtained results demonstrated the higher extraction capacity of NR-CF-Mag-MIP with recoveries between 97.6 and 101%. The limits of quantification (LOQs) for ME was 0.015 µg/L. Validation results on linearity, specificity, accuracy, precision, and stability, as well as on application to the analysis of samples taken up to 480 h after oral administration of 20 mg (two 10 mg capsules) of ME in healthy volunteers demonstrated the applicability to bioequivalence studies.