The activation of nuclear phosphoinositide 3-kinase C2β in all- trans-retinoic acid-differentiated HL-60 cells

• Published in: FEBS letters Vol. 529; no. 2; pp. 268 - 274
• Main Authors: Višnjić, Dora, Crljen, Vladiana, Ćurić, Josip, Batinić, Drago, Volinia, Stefano, Banfić, Hrvoje
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 09.10.2002
• Subjects: 1,25(OH)2D3, 1,25-dihydroxyvitamin D3; All- trans-retinoic acid; ATRA, all-trans-retinoic acid; db cAMP, dibutyryl-adenosine 3′, 5′-cyclic monophosphate; DMSO, dimethyl sulfoxide; EGFR, epidermal growth factor receptor; HL-60 cell; IFN-γ, interferon-γ; Nucleus; PDGFR, platelet-derived growth factor receptor; Phosphoinositide 3-kinase C2β; PI3K, phosphoinositide 3-kinase; PMA, phorbol 12-myristate 13-acetate; PtdIns(3,4)P2, phosphatidylinositol 3,4-bisphosphate; PtdIns(3,4,5)P3, phosphatidylinositol 3,4,5-trisphosphate; PtdIns(4,5)P2, phosphatidylinositol 4,5-bisphosphate; PtdIns, phosphatidylinositol; PtdInsP, phosphatidylinositol 3-phosphate; PtdInsP, phosphatidylinositol 4-phosphate; PtdSer, phosphatidylserine; Tyrosine phosphorylation; Phosphoinositide 3-kinase C2β; IFN-γ, interferon-γ; Nucleus; 1,25(OH) 2D 3, 1,25-dihydroxyvitamin D 3; PtdInsP, phosphatidylinositol 3-phosphate; All- trans-retinoic acid; PI3K, phosphoinositide 3-kinase; ATRA, all- trans-retinoic acid; PtdInsP, phosphatidylinositol 4-phosphate; db cAMP, dibutyryl-adenosine 3′, 5′-cyclic monophosphate; HL-60 cell; PtdIns(3,4,5)P 3, phosphatidylinositol 3,4,5-trisphosphate; PtdIns(3,4)P 2, phosphatidylinositol 3,4-bisphosphate; EGFR, epidermal growth factor receptor; PtdIns, phosphatidylinositol; PDGFR, platelet-derived growth factor receptor; Tyrosine phosphorylation; PtdSer, phosphatidylserine; DMSO, dimethyl sulfoxide; PtdIns(4,5)P 2, phosphatidylinositol 4,5-bisphosphate; PMA, phorbol 12-myristate 13-acetate
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/S0014-5793(02)03357-4

The activity of nuclear phosphoinositide 3-kinase C2β (PI3K-C2β) was investigated in HL-60 cells induced to differentiate along granulocytic or monocytic lineages. A significant increase in the activity of immunoprecipitated PI3K-C2β was observed in the nuclei and nuclear envelopes isolated from all- trans-retinoic acid (ATRA)-differentiated cells which was inhibited by the presence of PI3K inhibitor LY 294002. High-performance liquid chromatography analysis of inositol lipids showed an increased incorporation of radiolabelled phosphate in both PtdIns(3)P and PtdIns(3,4,5)P 3 with no changes in the levels of PtdIns(4)P, PtdIns(3,4)P 2 and PtdIns(4,5)P 2. Western blot analysis of the PI3K-C2β immunoprecipitates with anti-P-Tyr antibody revealed a significant increase in the level of the immunoreactive band corresponding to PI3K-C2β in the nuclei and nuclear envelopes isolated from ATRA-differentiated cells.