Luminescent material based on the [Eu(TTA)3(H2O)2] complex incorporated into modified silica particles for biological applications

• Published in: Journal of inorganic biochemistry Vol. 123; pp. 11 - 17
• Main Authors: Lourenço, Ana Valéria S., Kodaira, Cláudia A., Ramos-Sanchez, Eduardo M., Felinto, Maria Cláudia F.C., Goto, Hiro, Gidlund, Magnus, Malta, Oscar L., Brito, Hermi F.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2013
• Subjects: Bioassay; Biological Assay - methods; Europium - chemistry; Europium complex; Functionalized silica; Luminescence; Luminescence properties; Oxidized low density lipoprotein; Silicon Dioxide - chemistry; Functionalized silica; Oxidized low density lipoprotein; Bioassay; Luminescence properties; Europium complex
• ISSN: 0162-0134; 1873-3344
• DOI: 10.1016/j.jinorgbio.2013.02.006

Amino-functionalized luminescent silica particles were investigated for use in immunoassays. The particles were prepared by the Stöber method where the [Eu(TTA)3(H2O)2] complex (TTA: 3-thenoyltrifluoroacetonate) was incorporated into silica particles during the hydrolysis and condensation of TEOS: tetraethylorthosilicate. Then, the amino groups were introduced in the particle surface using APTS: 3-aminopropyltriethoxisilane. The resulting particles were characterized by scanning electron microscopy (SEM), X ray diffraction (XRD) and photoluminescence spectroscopy. In order to demonstrate the viability of the use of luminescent particles as optical markers, an enzyme–substrate reaction was performed using HRP: horseradish peroxidase. It was possible to verify the binding of HRP-oxidized LDL (low density lipoprotein) and anti-oxLDL antibody-luminescent silica particles through the evaluation of the presence of HRP. The bioassay data open a broad field for the development of protein-tagged luminescent particles for use in biomedical sciences. Luminescent marker prepared by the Stöber method, incorporating the [Eu(TTA)3(H2O)2] complex, as a potential candidate for development of the protocol for the quantification of the oxLDL. [Display omitted] •Amino-functionalized luminescent silica particles were prepared and characterized.•Viability of the use of luminescent particles as optical markers was examined.•Enzyme-substrate reaction was performed using HRP.•The bioassay data open a broad field for the biomedical sciences.