Effects of peritoneal fluid from endometriosis patients on the release of monocyte-specific chemokines by leukocytes

• Published in: Archives of gynecology and obstetrics Vol. 283; no. 6; pp. 1333 - 1341
• Main Authors: Na, Yong-Jin, Lee, Dong-Hyung, Kim, Seung-Chul, Joo, Jong-Kil, Wang, Ji-Won, Jin, Jun-O, Kwak, Jong-Young, Lee, Kyu-Sup
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.06.2011; Springer Nature B.V
• Subjects: Ascitic Fluid - immunology; CD4-Positive T-Lymphocytes - immunology; CD8-Positive T-Lymphocytes - immunology; Cells, Cultured; Chemokine CCL2 - metabolism; Chemokine CCL3 - metabolism; Chemokine CCL5 - metabolism; Chemokines; Chemokines, CC - metabolism; Endocrinology; Endometriosis; Endometriosis - immunology; Enzyme-Linked Immunosorbent Assay; Female; General Gynecology; Gynecology; Human Genetics; Humans; Leukocytes - immunology; Lymphocytes; Medicine; Medicine & Public Health; Monocytes - immunology; Neutrophils; Neutrophils - immunology; Obstetrics/Perinatology/Midwifery; Statistics as Topic; T-Lymphocytes - immunology; Endometriosis; Monocytes; CC chemokines; T cells; Neutrophils
• ISSN: 0932-0067; 1432-0711
• DOI: 10.1007/s00404-010-1583-1

Purpose Chemokines have been implicated in the pathological process of endometriosis. We compared the effects of peritoneal fluid obtained from patients with endometriosis (ePF) and controls without endometriosis (cPF) on the release of monocyte-specific CC chemokines such as monocyte chemotactic protein-1 (MCP-1), regulated upon activation normal T cell expressed and secreted (RANTES), and macrophage inflammatory protein-1α (MIP-1α) by neutrophils, monocytes, and T cells. Moreover, we evaluated the correlation between the levels of chemokines in ePF and their release by these cells. Methods Cells were obtained from healthy young volunteers and cultured with ePF ( n  = 12) or cPF ( n  = 8). The chemokine levels in the ePF and the supernatants of cultured cells with ePF were then measured by ELISA. Results There was a positive correlation between the levels of MCP-1 and MIP-1α in ePF. The addition of ePF to the cell cultures failed to increase the release of MCP-1, RANTES, and MIP-1α when compared to cPF, but the levels of RANTES in ePF were positively correlated with the release of RANTES by ePF-treated monocytes and T cells. Moreover, there was a positive correlation between the levels of RANTES and MIP-1α released by neutrophils and between the levels of MCP-1 and MIP-1α released by T cells. Finally, the levels of RANTES released by monocyte-derived macrophages and monocytes cultured with ePF were positively correlated. Conclusions These findings suggest that monocytes, neutrophils, and T cells release differential levels of MCP-1, RANTES, and MIP-1α in response to stimulation with ePF.