Identification of targeting peptides for the diagnosis of myocarditis

• Published in: Nanomedicine (London, England) Vol. 13; no. 7; pp. 787 - 801
• Main Authors: Martinez, Mario D, Trac, David Q, Brown, Milton E, Maher, Kevin O, Davis, Michael E
• Format: Journal Article
• Language: English
• Published: England Future Medicine Ltd 01.04.2018
• Subjects: Animals; Autoimmune Diseases - diagnosis; Autoimmune Diseases - diagnostic imaging; Autoimmune Diseases - physiopathology; Cardiomyopathy; Diagnostic Imaging; Discovery tools; Disease; Disease Models, Animal; Early Diagnosis; Echocardiography; Fluorescent Dyes - administration & dosage; Heart attacks; Heart failure; Humans; imaging; Inflammation; Ischemia; Laboratory animals; Libraries; Mice; myocarditis; Myocarditis - diagnosis; Myocarditis - diagnostic imaging; Myocarditis - physiopathology; Myosin Heavy Chains - isolation & purification; Myosin Heavy Chains - metabolism; Pediatrics; peptide; Peptide Library; Peptides; Peptides - isolation & purification; Peptides - metabolism; phage display; England; United States > US; peptide; phage display; imaging; myocarditis
• ISSN: 1743-5889; 1748-6963
• DOI: 10.2217/nnm-2018-0023

Current diagnostic tests for myocarditis are invasive and have low diagnostic value. Our aim was to identify potential targeting peptides to detect early myocarditis following intravenous delivery. We used an animal model of experimental autoimmune myocarditis and a phage display library to identify potential targeting peptides. After several steps, we selected two peptides, MyH-PhD-05 and MyH-PhD-120, for screening using fluorescent imaging. Immunofluorescence and proteonomic analysis was used to identify potential cellular and molecular targets of MyH-PhD-05. Echocardiography was used to assess functional changes. Peptide MyH-PhD-05 was able to detect animals with severe myocarditis even in the absence of functional changes. Immunofluorescence demonstrated that MyH-PhD-05 colocalizes with CD4 T cells and monocytes (CD11b ) in cardiac infiltrates. We identified potential targeting peptides for the diagnosis of myocarditis. Future studies will focus on better identification of potential targets and translating this technology to clinically relevant imaging modalities.