Differential expression of miR-let7a in hair follicle cycle of Liaoning cashmere goats and identification of its targets

• Published in: Functional & integrative genomics Vol. 18; no. 6; pp. 701 - 707
• Main Authors: Ma, Tao, Li, Jianping, Jiang, Qian, Wu, Sufang, Jiang, Huaizhi, Zhang, Qiaoling
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.11.2018; Springer Nature B.V
• Subjects: Animal Genetics and Genomics; Animals; Biochemistry; Bioinformatics; Biomedical and Life Sciences; c-Myc protein; Caprinae; Cashmere; Cell Biology; Fibroblast growth factor 5; Fibroblast Growth Factor 5 - genetics; Gene expression; Gene Expression Profiling; Gene Expression Regulation - genetics; Gene regulation; Goats - genetics; Goats - growth & development; Hair; Hair Follicle - growth & development; Hair Follicle - metabolism; Hair loss; Insulin-like growth factors; Life Sciences; Microbial Genetics and Genomics; MicroRNAs - genetics; miRNA; Myc protein; Original Article; Plant Genetics and Genomics; Proto-Oncogene Proteins c-myc - genetics; Receptor, IGF Type 1 - genetics; Reporter gene; Transcriptome - genetics; Liaoning cashmere goats; Differential expression; Hair follicle development; miR-let7a
• ISSN: 1438-793X; 1438-7948
• DOI: 10.1007/s10142-018-0616-x

In order to improve the production and quality of Chinese cashmere, the research of hair follicle development has aroused more and more attention; the regulation mechanism of miRNA in hair follicle development has become a hot spot. A survey of transcriptome profiling screened 10 hair follicle-related miRNAs that were differentially expressed, including miR-let7a. In this study, the expression of miR-let7a was lower in anagen of hair follicle of cashmere goats than that in catagen of hair follicle of cashmere goats ( p  < 0.01). Results were in accordance with transcriptome data. The expression patterns of miR-let7a target genes (IGF-1R, C-myc, and FGF5) were verified by qRT-PCR, which were consistent with the results of Western blot and showed a downward trend. The dual-luciferase reporter gene system was used to verify the correlation between the expression of miR-let7a and its target genes, and it showed that miR-let7a negatively correlates with C-myc and FGF5. Present study offers new information on miRNAs and their related target genes in the regulation of hair follicle development mechanism.