Circular RNA circASS1 is downregulated in breast cancer cells MDA-MB-231 and suppressed invasion and migration

• Published in: Epigenomics Vol. 11; no. 2; pp. 199 - 213
• Main Authors: Hou, Jun-chen, Xu, Zhi, Zhong, Shan-liang, Zhang, He-da, Jiang, Lin-hong, Chen, Xiu, Zhu, Ling-ping, Li, Jian, Zhou, Si-ying, Yang, Su-jin, He, Yun-jie, Wang, Dan-dan, Deng, Fei, Zhang, Qian, Wang, Jin-yan, Hu, Jia-hua, Zhang, Wei, Wu, Yang, Ding, Li, Zhao, Jian-hua, Tang, Jin-hai
• Format: Journal Article
• Language: English
• Published: England Future Medicine Ltd 01.02.2019
• Subjects: Breast cancer; breast carcinoma; Breast Neoplasms - genetics; Cancer; Cell cycle; Cell Line, Tumor; Cell Movement; circASS1; circRNA; Circular RNA; Circularity; Deoxyribonucleic acid; DNA; DNA methylation; DNA microarrays; Down-Regulation; Epigenetics; Female; Gene expression; Humans; Hybridization; invasion; Kinases; MCF-7 Cells; MDA-MB-231; metastasis; MicroRNAs; MicroRNAs - genetics; MicroRNAs - metabolism; migration; miRNA; Regulation; Ribonucleic acid; RNA; RNA, Circular - genetics; RNA, Circular - metabolism; Western blotting; Wound healing; United States > US; China; RNA; circRNA; invasion; breast carcinoma; metastasis; circular RNA; migration; breast cancer; circASS1; MDA-MB-231
• ISSN: 1750-1911; 1750-192X
• DOI: 10.2217/epi-2017-0167

The study aimed to investigate the role of circular RNA circASS1 in breast cancer cells. Circular RNAs microarray expression profile were analyzed in MCF-7, MDA-MB-231, and qRT-PCR and western blotting were used to quantify expression of circASS1 and its parental gene . Wound healing, migration and invasion assay were performed. Luciferase assay system was used to detect harbored miRNA. CircASS1 in MDA-MB-231 is downregulated comparing to MCF-7, and overexpression of circASS1 could suppress invasion and migration. While silence, it could promote invasion and migration. MiR-4443 functioning as a tumor promoter gene could be captured by circASS1. ASS1 is upregulated in loss-of-function experiments, while downregulated in gain-of-function experiments. CircASS1 suppresses invasion and migration capacity of breast cancer cells and harbored miR-4443.