Maternal circadian disruption before pregnancy impairs the ovarian function of female offspring in mice

• Published in: The Science of the total environment Vol. 864; p. 161161
• Main Authors: Guan, Yajie, Xu, Manlin, Zhang, Zhe, Liu, Chuanming, Zhou, Jidong, Lin, Fei, Fang, Junshun, Zhang, Yang, Yue, Qiuling, Zhen, Xin, Yan, Guijun, Sun, Haixiang, Liu, Wenwen
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.03.2023
• Subjects: Animals; Circadian Clocks - genetics; Circadian Rhythm - physiology; Clock genes; Female; Female fertility; Immune; Lipid metabolism; Maternal circadian disruption; Mice; Mice, Inbred ICR; Obesity; Ovary; Pregnancy; Reproduction; IHC; Bdnf; Fshb; Bmper; Cry1; HRP; Flt4; VAT; Clock genes; FPKM; Ovary; Dbp; Srd5a1; Fshr; Ltbp2; FSH; Scd1; Lipid metabolism; Cyp; Crhr1; Hsl; Sfrp5; Female fertility; Ck1Ɛ; Per1/Per2; Cpt1; Lhcgr; PCOS; Dgat2; Ror; Dgat1; ROS; IL-17; IVF; NOXs; VO; Maternal circadian disruption; HTF; ELISA; Kiss1; DDX4; SAT; Lhb; Gnrh1; Lpl; RIPA; Bmal1; Nr1d1/Nr1d2; HDL; DEGs; Cxcl9; LDL; HPO axis; Immune; Atgl; RIA; ECLIA; TC; Mcd; Lyve1; TG; Usp53; Lal; Fas; LH
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2022.161161

Circadian disturbance brought on by shift employment, nighttime light pollution, and other factors is quite prevalent in contemporary culture. However, the effect of maternal circadian disruption before pregnancy on the reproduction of offspring in mice requires further research. Herein, we exposed female ICR mice to constant light to establish a model of preconceptional circadian disruption and then checked the ovarian function of female offspring (named the CLE group below). Our results revealed obesity, abnormal lipid metabolism and earlier puberty onset in the CLE group. Additionally, impaired ovarian follicle development, oocyte quality and preimplantation embryo development were shown in the CLE group. Moreover, the expression levels of Gnrh1 in the hypothalamus and Cyp17a1, Bmper, Bdnf and Lyve1 in ovaries, as well as circadian clock genes, including Clock, Cry1, Nr1d2 and Per2, were significantly downregulated in the CLE group. Mechanistically, immune responses, including the interleukin-17 (IL-17) signalling pathway, cytokine–cytokine receptor interaction and the chemokine signalling pathway, were altered in the CLE group, which may be responsible for the damaged ovarian function. [Display omitted] •Maternal circadian disruption before pregnancy damaged the ovarian function in female offspring.•The disorder of circadian rhythm before pregnancy resulted in abnormal lipid metabolism of female offspring.•Maternal circadian rhythm disorder before pregnancy caused changes in the immune microenvironment of offspring ovaries.