Single cell methods for methane oxidation analysis

• Published in: Methods in enzymology Vol. 495; p. 149
• Main Authors: Konopka, Michael C, McQuaide, Sarah, Ojala, David S, Kalyuzhnaya, Marina G, Lidstrom, Mary E
• Format: Journal Article
• Language: English
• Published: United States 2011
• Subjects: Equipment Design; Flow Cytometry - instrumentation; Flow Cytometry - methods; Methane - metabolism; Methylococcaceae - metabolism; Methylosinus - metabolism; Microbiological Techniques - instrumentation; Microbiological Techniques - methods; Oxidation-Reduction; Single-Cell Analysis - instrumentation; Single-Cell Analysis - methods
• ISSN: 1557-7988
• DOI: 10.1016/B978-0-12-386905-0.00010-3

Respiration is widely used for evaluation of the metabolic capabilities or physiological state of the microbial culture. This chapter describes novel approaches for characterization of respiration at a single cell level: (1) flow cytometry-based redox sensing (FCRS) of actively metabolizing microbes; (2) respiration response imaging (RRI) for real-time detection of substrate stimulated redox responses of individual cells; (3) respiration detection system: microobservation chamber (RDS: MC), a single cell analysis system for carrying out the physiological and genomic profiling of cells capable of respiring C(1) compounds. The techniques are suitable for description of physiological heterogeneity among cells in a single microbial population and could be used to characterize distribution of methylotrophic ability among microbial cells in the natural environmental samples.