Analysis of antibiotic resistance genes reveals their important roles in influencing the community structure of ocean microbiome

• Published in: The Science of the total environment Vol. 823; p. 153731
• Main Authors: Yang, Pengshuo, Hao, Shiguang, Han, Maozhen, Xu, Junjie, Yu, Shaojun, Chen, Chaoyun, Zhang, Houjin, Ning, Kang
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.06.2022
• Subjects: Antibiotic resistance gene; Data-mining; Human impact; Marine microbiome; Human impact; Data-mining; Antibiotic resistance gene; Marine microbiome
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2022.153731

Antibiotic resistance gene (ARG) content is a well-established driver of microbial abundance and diversity in an environment. By reanalyzing 132 metagenomic datasets from the Tara Oceans project, we aim to unveil the associations between environmental factors, the ocean microbial community structure and ARG contents. We first investigated the structural patterns of microbial communities including both prokaryotes such as bacteria and eukaryotes such as protists. Additionally, several ARG-dominant horizontal gene transfer events between Protist and Prokaryote have been identified, indicating the potential roles of ARG in shaping the ocean microbial communities. For a deeper insight into the role of ARGs in ocean microbial communities on a global scale, we identified 1926 unique types of ARGs and discovered that the ARGs are more abundant and diverse in the mesopelagic zone than other water layers, potentially caused by limited resources. Finally, we found that ARG-enriched genera were often more abundant compared to their ARG-less neighbors in the same environment (e.g. coastal oceans). A deeper understanding of the ARG-microbiome relationships could help in the conservation of the oceanic ecosystem. [Display omitted] •The distribution of ocean microbiome was investigated on the global scale.•ARGs were dominated in the gene communications between Protist and Prokaryote.•In the same community, ARG-enriched genera outnumbered ARG-less genera.•Impact of ARGs and environmental factors on microbiome were quantified.•Human activities may influence the ARGs and hence ocean microbial communities.