Inhibition of Adipocyte Differentiation by cMyc Is Not Accompanied by Alterations in Cell Cycle Control

Using a preadipocyte cell line constitutively expressing cMyc, we set out to determine if the ability of cMyc to inhibit adipogenic differentiation was functionally distinct from its role in cell cycle progression and transformation. We now report that in this system differentiation control and cell...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 269; no. 2; pp. 438 - 443
Main Authors Heath, Victoria J., Gillespie, David A.F., Crouch, Dorothy H.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 16.03.2000
Subjects
3T3 Cells
Adipocytes - cytology
adipogenesis
Animals
c-Myc protein
C/EB-a protein
Cell Cycle
Cell Differentiation - physiology
Flow Cytometry
Mice
Myc
Proto-Oncogene Proteins c-myc - physiology
cell cycle
Myc
adipogenesis
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Summary:Using a preadipocyte cell line constitutively expressing cMyc, we set out to determine if the ability of cMyc to inhibit adipogenic differentiation was functionally distinct from its role in cell cycle progression and transformation. We now report that in this system differentiation control and cellular transformation are separable functions of cMyc. Furthermore, the Myc-induced inhibition of adipocyte differentiation appears to be mediated via suppression of C/EBP-α and p21 gene expression late in the adipogenic differentiation programme, without deregulation of either cell cycle control or gene expression during the early stages of the process.
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ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2000.2316