Cytotoxic steroids from the stems of Strophanthus divaricatus

• Published in: Phytochemistry (Oxford) Vol. 210; p. 113668
• Main Authors: Ran, Hong-Ling, Huang, Sheng-Zhuo, Wang, Hao, Yang, Li, Gai, Cui-Juan, Duan, Rui-Jun, Dai, Hao-Fu, Guan, Ya-Li, Mei, Wen-Li
• Format: Journal Article
• Language: English
• Published: OXFORD Elsevier Ltd 01.06.2023; Elsevier
• Subjects: Antineoplastic Agents; Apocynaceae; Biochemistry & Molecular Biology; C21 pregnane; Cardiac glycosides; Cell Line, Tumor; Cytotoxicity; Glycosides - chemistry; Humans; Life Sciences & Biomedicine; Molecular Structure; Plant Sciences; Pregnanes - chemistry; Pregnanes - pharmacology; Science & Technology; Strophanthus; Strophanthus divaricatus; Apocynaceae; Strophanthus divaricatus; Cytotoxicity; C21 pregnane; Cardiac glycosides; GLYCOSIDES; C pregnane; CARDENOLIDES; PROLIFERATION; CELL
• ISSN: 0031-9422; 1873-3700
• DOI: 10.1016/j.phytochem.2023.113668

Phytochemical investigation on the stems of Strophanthus divaricatus led to the isolation of four undescribed cardiac glycosides and one undescribed C21 pregnane, together with eleven known steroids. Their structures were elucidated by a comprehensive analysis of HRESIMS, 1D and 2D NMR spectra. The absolute configuration of 16 was determined by comparison of the experimental and computed ECD spectra. Compounds 1–13 and 15 displayed potent to significant cytotoxicity against human cancer cell lines K562, SGC-7901, A549 and HeLa with IC50 values of 0.02–16.08, 0.04–23.13, 0.06–22.31 and 0.06–15.13 μM, respectively. Four undescribed cytotoxicity cardiac glycosides and one undescribed C21 pregnane were isolated from stems of Strophanthus divaricatus. [Display omitted] •Five new cardiac steroids were isolated from Strophanthus divaricatus.•The absolute configuration of 16 was determined by ECD spectra.•Fifteen cardiac glycosides exhibited significant cytotoxicity.