Conditions of cultivation, composition, and biological activity of mycelium of Flammulina velutipes (Fr.) P. Karst

• Published in: Applied biochemistry and microbiology Vol. 46; no. 5; pp. 536 - 539
• Main Authors: Kozhemyakina, N. V, Ananyeva, E. P, Gurina, S. V, Galynkin, V. A
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : SP MAIK Nauka/Interperiodica 01.09.2010; SP MAIK Nauka/Interperiodica; Springer Nature B.V
• Subjects: Biochemistry; Biomedical and Life Sciences; Carbohydrates; Cells; Cultivars; Flammulina velutipes; Karst; Life Sciences; Medical Microbiology; Microbiology; Solution chemistry; Xylose; Fruit Body; Biomass Output; Apply Biochemistry; Fucose
• ISSN: 0003-6838; 1608-3024
• DOI: 10.1134/S0003683810050121

A study is made on a strain of higher basydiomycete Flammulia velutipes (Fr.) P. Karst. The conditions of maximum biomass production by Flammulia velutipes were studied. Soluble and insoluble fractions were isolated from mycelium. The composition of cultured mycelium and aqueous extracts from mycelium were investigated. These objects mainly contained carbohydrates (65.3 and 84.0% in insoluble and soluble fractions, respectively, and 56% mycelium), proteins (7.5-10.0% in fractions and 17.5% in mycelium), as well as an insignificant amount of mineral substances. The main carbohydrate component of fractions was glucose (53.6-78.8%); galactose and mannose were also present, as well as fucose and xylose in insignificant amounts. The aqueous extracts from mycelium demonstrated immunomodulating activity. They rendered a stimulating effect on the functional activity of macrophages—central cells of the reticluoendothelial system. The soluble fraction had a more pronounced effect than the insoluble fraction.