A Non-invasive, in Vivo Technique for Monitoring Vascular Status of Glioblastoma during Angiogenesis

• Published in: Technology in cancer research & treatment Vol. 6; no. 6; pp. 641 - 650
• Main Authors: Saxena, Vishal, Gonzalez-Gomez, Ignacio, Laug, Walter E
• Format: Journal Article
• Language: English
• Published: Los Angeles, CA SAGE Publications 01.12.2007; Adenine Press
• Subjects: Algorithms; Animals; Biological and medical sciences; Brain Neoplasms - blood supply; Brain Neoplasms - metabolism; Cell Line, Tumor; Female; Glioblastoma - blood supply; Glioblastoma - metabolism; Humans; Magnetic Resonance Imaging; Medical sciences; Mice; Mice, Nude; Models, Theoretical; Neovascularization, Pathologic - diagnosis; Neurology; Optics and Photonics; Oxyhemoglobins - analysis; Spectroscopy, Near-Infrared - methods; Tumors of the nervous system. Phacomatoses; Water - analysis; Optical absorption spectroscopy; Angiogenesis; Laser optics; Physiological Optics; Brain Tumor; Glioblastoma; Tissue Optics; Hemodynamics; Noninvasive imaging; Vascularization; Applied physics; Fiber optics; Fiber; Malignant glioma; Spectrometry; Absorption; Blood vessel; Invasive cancer; Neovascularization; Nervous system diseases; Nutrition; and Fiber optics; Exploration; Malignant tumor; Intracranial tumor; Feeding; Cerebral disorder; In vivo; Diet; Surveillance; Central nervous system disease; Laser; Medical imagery; Circulatory system; Technique; Cancer
• ISSN: 1533-0346; 1533-0338
• DOI: 10.1177/153303460700600608

The growth of solid tumors dependent on the process of angiogenesis in which growth factors secreted by tumor and stromal cells promote endothelial cell proliferation, migration, and maturation. This process generates a tumor-specific vascular supply and enables small or dormant tumors to grow rapidly with exponential increases in tumor volume. Determination of tumor oxygenation at the microvascular level will provide important insight into tumor growth, angiogenesis, necrosis, and therapeutic response, and will facilitate to develop protocols for studying tumor behavior. A non-invasive multi-modality approach based on near infrared spectroscopy (NIRS) technique, namely: Steady State Diffuse Optical Spectroscopy (SSDOS) along with Magnetic Resonance Imaging (MRI) is applied for monitoring the concentration of oxyhemoglobin, deoxyhemoglobin and water within tumor region and for studying the vascular status of tumor and the patho-physiological changes that occur during angiogenesis. Since, the growth of solid tumors depends on the formation of new blood vessels, an association between intramural microvessel density (MVD) and tumor oxygenation is also investigated. The relative decrease in oxygenation value with tumor growth indicates that though blood vessels infiltrate and proliferate the tumor region, a hypoxic trend is clearly present.