Functional Cross-Talk of HIV-1 Tat with p53 through Its C-Terminal Domain

• Published in: Biochemical and biophysical research communications Vol. 287; no. 2; pp. 556 - 561
• Main Authors: Ariumi, Yasuo, Kaida, Atsushi, Hatanaka, Masakazu, Shimotohno, Kunitada
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 21.09.2001
• Subjects: AIDS; Animals; CBP/p300; COS Cells; Gene Expression - drug effects; Gene Expression - physiology; Gene Products, tat - chemistry; Gene Products, tat - pharmacology; Gene Silencing; HIV Long Terminal Repeat - physiology; HIV-1; HIV-1 - chemistry; HIV-1 - physiology; Human immunodeficiency virus; NF-kappa B - metabolism; NF-κB; p53; p53 protein; Protein Structure, Tertiary; replication; Tat; tat Gene Products, Human Immunodeficiency Virus; tat protein; Transcriptional Activation - drug effects; Tumor Suppressor Protein p53 - genetics; Tumor Suppressor Protein p53 - metabolism; Virus Replication; HIV-1; replication; NF-κB; Tat; CBP/p300; AIDS; p53
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.2001.5626

Human immunodeficiency virus type 1 (HIV-1) Tat repressed the p53-dependent gene expression through its C-terminal domain of Tat (amino acid residues 73–86) independent of the involvement of NF-κB and coactivator CBP/p300. Although Tat did not directly bind to p53, this repression required the N-terminal domain of p53. In contrast, Tat and p53 cooperated in the activation of HIV-1 gene expression. Thus, the cross-talk between Tat and p53 may be linked with cellular transformation by HIV-1 infection or activation of HIV-1 replication.