Suppression of facilitative glucose transporter 1 mRNA can suppress tumor growth

• Published in: Cancer letters Vol. 154; no. 2; pp. 175 - 182
• Main Authors: Noguchi, Yoshikazu, Saito, Aya, Miyagi, Yohei, Yamanaka, Shoji, Marat, Doulet, Doi, Chiharu, Yoshikawa, Takaki, Tsuburaya, Akira, Ito, Takaaki, Satoh, Shinobu
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 30.06.2000; Elsevier
• Subjects: Adenocarcinoma - metabolism; Animals; Antineoplastic agents; Antisense; Apoptosis; Biological and medical sciences; Biological Transport - genetics; Chemotherapy; DNA Fragmentation; DNA, Antisense - metabolism; DNA, Complementary - metabolism; Dose-Response Relationship, Drug; Flow Cytometry; G1 Phase; Glucose transporter 1; Glucose Transporter Type 1; Glucose uptake; Kinetics; Medical sciences; Mice; Mice, Inbred ICR; Mice, Nude; Monosaccharide Transport Proteins - genetics; Monosaccharide Transport Proteins - metabolism; Neoplasm Transplantation; Neoplasms, Experimental - pathology; Pharmacology. Drug treatments; Proto-Oncogene Proteins p21(ras) - biosynthesis; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - metabolism; S Phase; Stomach Neoplasms - metabolism; Suppression, Genetic; Time Factors; Transfection; Tumor Cells, Cultured; Glucose transporter 1; Antisense; Glucose uptake; Antineoplastic agent; Human; Cell proliferation; Stomach; Glucose; Malignant tumor; Antisense oligonucleotide; In vitro; Biological activity; Messenger RNA; Transfection; Established cell line; Digestive diseases; Antisense DNA; Inhibition; Gene therapy; Carrier protein; Gastric disease; Facilitated transport
• ISSN: 0304-3835; 1872-7980
• DOI: 10.1016/S0304-3835(00)00392-X

We attempted to suppress glucose transporter 1 (GLUT1) expression by transfecting MKN45 cells with cDNA for antisense GLUT1. Glucose transport was significantly decreased in cells with antisense GLUT1 compared with wild-type cells or cells with vector alone. Suppression of GLUT1 mRNA resulted in a decreased number of cells in the S phase. This was accompanied by overexpression of p21 protein. Tumorigenicity in the nude mice injected with antisense GLUT1 expressing cells was significantly slower than in those with wild-type MKN45 cells. These results suggest that antisense GLUT1 mRNA inhibits tumor growth through a G 1 arrest and that expression of antisense GLUT1 mRNA via gene therapy can be used as a tool in the treatment of cancer.