Are mulch biofilms used in agriculture an environmentally friendly solution? - An insight into their biodegradability and ecotoxicity using key organisms in soil ecosystems

• Published in: The Science of the total environment Vol. 828; p. 154269
• Main Authors: Ferreira-Filipe, Diogo A., Paço, Ana, Natal-da-Luz, Tiago, Sousa, José Paulo, Saraiva, Jorge A., Duarte, Armando C., Rocha-Santos, Teresa, Patrício Silva, Ana L.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.07.2022
• Subjects: Agriculture; Animals; Biodegradable Plastics; Biofilms; Earthworms; Ecosystem; Fungi; Microplastics; Oligochaeta; Plastics; Plastics biodegradation; Soil; Soil ecotoxicology; Soil Pollutants - toxicity; Soil ecotoxicology; Fungi; Plastics biodegradation; Biofilms; Earthworms
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2022.154269

Biobased and biodegradable plastic mulch films (aka, mulch biofilm) have emerged as a sustainable alternative to conventional plastic mulch films in agriculture, promising to reduce soil contamination with plastic residues through in situ biodegradation. However, current standards certifying biodegradable plastics cannot predict biodegradability in natural settings. The scarce studies considering the possible biodegradation and ecotoxicity of mulch biofilms in soil systems question the environmental friendliness of these alternative options. This study assessed the biodegradation of a commercially available mulch biofilm by the soil-dwelling fungus Penicillium brevicompactum (in solid culture media and soil for 15 and 28 days, respectively), and the ecotoxicological effects of mulch biofilm microplastics on the earthworm Eisenia andrei (pristine or UV-weathered, at 0.125–0.250–0.500 g/kg). Results (from microplastics' mass loss, microscopy, and FTIR spectroscopy) suggest that the presence of P. brevicompactum promotes mulch biofilm's biodegradation. Exposure to environmental concentrations of pristine biofilm microplastics (and its ingestion) increased earthworms' sensitivity to touch, induced physiological alterations, decreased energy reserves, and decreased their reproduction (>30%). Conversely, exposure to weathered biofilm microplastics slightly increased earthworms' sensitivity, as well as carbohydrate reserves,without affecting their reproduction. The tested mulch biofilm seems to be, at first sight, an environmentally friendly alternative as it presented susceptibility for biodegradation by a widespread fungus, and the absence of ecotoxicological chronic effects on a key macroinvertebrate species in soil ecosystems when considering environmental relevant concentrations and plastics weathered conditions. Notwithstanding, the obtained results highlight the need to revise current standards, as they often neglect the role of, and their chronic effects on, naturally occurring organisms. [Display omitted] •Biodegradation and ecotoxicity of a certified mulch biofilm (MB) in soils were assessed.•MB seemed to biodegrade in the presence of Penicillium brevicompactum.•Pristine MB microplastics induced physiological and chronic effects on Eisenia andrei.•Weathered MB microplastics had no chronic effects on Eisenia andrei.•Results highlight the need to revaluate standards certifying biodegradable plastics.