Oxygen tension imaging in the mouse retina

A newly developed microscope-based imaging system was used to measure the oxygen tension (PO2) inside the retinal and choroidal vessels of mice and to generate in vivo maps of retinal PO2. These maps were generated from the phosphorescence lifetimes of an injected palladium-porphyrin compound using...

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Bibliographic Details
Published inAnnals of biomedical engineering Vol. 31; no. 9; pp. 1084 - 1096
Main Authors Shonat, Ross D, Kight, Amanda C
Format Journal Article
LanguageEnglish
Published United States Springer Nature B.V 01.10.2003
Subjects
Animals
Diabetic retinopathy
Equipment Failure Analysis
Female
Hypoxia
Macular degeneration
Mesoporphyrins
Metalloporphyrins
Mice
Mice, Inbred C57BL
Microscopy, Fluorescence - instrumentation
Microscopy, Fluorescence - methods
Oxygen
Oxygen - analysis
Oxygen - metabolism
Oxygen Consumption - physiology
Palladium
Pressure
Reproducibility of Results
Retina - cytology
Retina - metabolism
Retinal Vessels - cytology
Retinal Vessels - metabolism
Sensitivity and Specificity
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Summary:A newly developed microscope-based imaging system was used to measure the oxygen tension (PO2) inside the retinal and choroidal vessels of mice and to generate in vivo maps of retinal PO2. These maps were generated from the phosphorescence lifetimes of an injected palladium-porphyrin compound using a frequency-domain measurement. The system was fully calibrated and used to produce retinal PO2 maps at different inspiratory oxygen fractions. PO2 rose accordingly and predictably as inspiratory O2 was stepped from hypoxic to hyperoxic conditions. Important experimental and acquisition parameters necessary for applying phosphorescence lifetime imaging to the mouse eye were investigated, including camera exposure and intensifier gain settings. Because of a need to limit light exposure to the retina, PO2 map quality as measured by the coefficient of determination was investigated as a function of signal-to-noise and accumulated excitation energy deposition. With the development of this technology for use in mice, the potential for investigating the oxygen dynamics in genetically engineered mouse models of retinal disease, including diabetic retinopathy, glaucoma, and age-related macular degeneration, is advanced.
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ISSN:0090-6964
1573-9686
DOI:10.1114/1.1603256