Extracellular proteolytic activity of bacteria from soda-salt lakes of Transbaikalia
Influence of nitrogen source on proteinases synthesis in aerobic alkalotolerant and halotolerant bacteria from soda-salt lakes of Transbaikalia was studied. Maximal accumulation of proteinases was revealed on medium with peptones. Introduction of various sources of nitrogen in the medium did not res...
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Published in | Applied biochemistry and microbiology Vol. 46; no. 6; pp. 580 - 585 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Dordrecht : SP MAIK Nauka/Interperiodica
01.11.2010
SP MAIK Nauka/Interperiodica Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Influence of nitrogen source on proteinases synthesis in aerobic alkalotolerant and halotolerant bacteria from soda-salt lakes of Transbaikalia was studied. Maximal accumulation of proteinases was revealed on medium with peptones. Introduction of various sources of nitrogen in the medium did not result in increase of enzyme activity in cultural liquid. It was indicated that secreting proteinases of the studied bacteria strains possess narrow substrate specificity, hydrolyze proteins and n-nitroanilide substrates have maximal activity during GlpAALpNA hydrolysis. Data of inhibitory analysis and substrate specificity of studied extracellular enzymes indicate that they belong to a class of serine proteinases of subtilisin-like type. |
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Bibliography: | http://dx.doi.org/10.1134/S0003683810060049 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0003-6838 1608-3024 |
DOI: | 10.1134/S0003683810060049 |