Sources of Reactive Oxygen Species Production in Excitotoxin-Stimulated Cerebellar Granule Cells
Reactive oxygen species (ROS) production in rat cerebellar granule cells in the presence of the excitotoxinsN-methyl-d-aspartate (NMDA) and kainic acid (KA) and by the protein kinase C activator phorbol myristate acetate (PMA) was Ca2+-dependent and resulted in decreased cell viability. Exposure of...
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Published in | Biochemical and biophysical research communications Vol. 256; no. 2; pp. 320 - 324 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
16.03.1999
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Subjects | |
Online Access | Get full text |
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Summary: | Reactive oxygen species (ROS) production in rat cerebellar granule cells in the presence of the excitotoxinsN-methyl-d-aspartate (NMDA) and kainic acid (KA) and by the protein kinase C activator phorbol myristate acetate (PMA) was Ca2+-dependent and resulted in decreased cell viability. Exposure of stimulated cells to rotenone (a respiratory chain inhibitor) did not decrease ROS levels and did not affect short-term cell viability. In cells stimulated by NMDA and KA, exposure to indomethacin (a cyclooxygenase inhibitor) and nialamide (a monoamine oxidase inhibitor) caused a decrease in ROS levels and increased cell viability occurred in NMDA-treated cells. In contrast, PMA-stimulated neurons did not show decreased ROS levels when exposed to indomethacin and nialamide. These studies suggest that there is a multiplicity of routes for Ca2+-dependent ROS production in neurons but that ROS generation by cyclooxygenase and monoamine oxidase is not controlled by protein kinase C. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0006-291X 1090-2104 |
DOI: | 10.1006/bbrc.1999.0325 |