Antioxidant activity of oxygen-containing aromatic compounds

• Published in: Applied biochemistry and microbiology Vol. 47; no. 4; pp. 346 - 355
• Main Authors: Potapovich, M. V, Kurchenko, V. P, Metelitza, D. I, Shadyro, O. I
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer-Verlag 01.07.2011; SP MAIK Nauka/Interperiodica; Springer Nature B.V
• Subjects: antioxidant activity; Antioxidants; Aromatic compounds; Biochemistry; Biomedical and Life Sciences; caffeic acid; dimethyl sulfoxide; Enzymes; Free radicals; Hydrogen peroxide; Life Sciences; Medical Microbiology; Microbiology; Oxygen; Phenols; syringic acid; Caffeic Acid; Guaiacol; Hemin; Apply Biochemistry; Syringic Acid
• ISSN: 0003-6838; 1608-3024
• DOI: 10.1134/S0003683811040144

Inhibition efficiency (antioxidant activity) of 26 oxygen-containing aromatic compounds was studied in methemalbumin-H2O2-o-phenylenediamine (PDA) or tetramethylbenzidine (TMB) pseudoperoxidase system at 20°C in buffered physiological solution (pH 7.4) containing 6% DMF and 0.25% DMSO. The inhibitor’s efficiency was quantitatively characterized by the inhibition constants (K i, μM) or the inhibition degree (%). K i values varied in the range of 4 to 500 μM and were influenced by a substrate, the structure of an inhibitor, hydroxyl groups, electron-donating substituents in aromatic ring, and steric hindrances. The type of inhibition at cooxidation of eight pairs was noncompetitive, and that of five pairs was mixed and determined by the substrate nature and the inhibitor structure. Lignin phenolic compounds of guaiacyl and syringal series exhibited high antioxidant activity (K i in the range of 10–300 μM), and their efficiency decreased in the following order: caffeic acid > synapaldehyde > syringic acid > coniferyl aldehyde > para-hydroxycoumaric acid.