Pyrethroid susceptibility status and functional analysis of cytochrome P450 CYP6 subfamily genes in field Aedes albopictus, in Jiangsu, China

• Published in: Journal of Asia-Pacific entomology Vol. 27; no. 1; pp. 102210 - 36
• Main Authors: Zou, Feifei, Guo, Qin, Shen, Bo, Lv, Yuan
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.03.2024; 한국응용곤충학회
• Subjects: adults; Aedes albopictus; arboviruses; biomarkers; China; cluster analysis; CYP6; cytochrome P-450; deltamethrin; dengue; entomology; Field strain; genomics; insecticide resistance; larvae; mosquito control; multigene family; pyrethrins; Resistance; resistance management; RNA; species; subfamily; 농학; China; Resistance; Field strain; Aedes albopictus; CYP6
• ISSN: 1226-8615; 1876-7990; 1876-7790
• DOI: 10.1016/j.aspen.2024.102210

[Display omitted] •First revealing Ae. albopictus had DM resistance in Jiangsu, China.•Combining comparative genomics and CYP6 clusters to find DM resistance genes.•The significance of three CYP6 genes in DM resistance in all field mosquitoes.•Knockdown of three genes increased the sensitivity of mosquitoes to DM in vivo. Aedes albopictus with a worldwide distribution, has been a vector for more than 20 arboviruses such as dengue, chikugunya, Zika and West Nile, but insecticide resistance in A. albopictus has become more and more serious, as a result of improper application of pyrethroid insecticides for mosquito control. The P450 gene cluster is known to be involved in metabolic mechanisms of pyrethroid resistance in other mosquito species. However, little is known about metabolic resistance in field A. albopictus in China. In this study, we collected five different field strains and first revealed the larval and adult resistance status of A. albopictus to deltamethrin in Jiangsu province of China. A combination of comparative genomics and P450 gene cluster analysis was conducted to investigate the underlying CYP6-mediated metabolic resistance mechanisms against deltamethrin in these field mosquitoes. The significance of three CYP6 genes, AALF024122, AALF024124 and AALF018944 in deltamethrin resistance was identified in our study. Furthermore, RNA interference-mediated silencing of three genes all increased the sensitivity of mosquitoes to deltamethrin in vivo, showing that these genes may be viable biomarkers for pyrethroid resistance management in field A. albopictus.