New insights of CRISPR technology in human pathogenic fungi

• Published in: Future microbiology Vol. 14; no. 14; pp. 1243 - 1255
• Main Authors: Román, Elvira, Prieto, Daniel, Alonso-Monge, Rebeca, Pla, Jesús
• Format: Journal Article
• Language: English
• Published: England Future Medicine Ltd 01.09.2019
• Subjects: Candida albicans - genetics; Chromosomes; CRISPR; CRISPR-Associated Protein 9 - genetics; CRISPR-Cas Systems; Deoxyribonucleic acid; DNA; double-strand break; Enzymes; fungal genetics; Fungal infections; Fungi; Fungi - genetics; Fungi - pathogenicity; Gene Editing; Gene expression; gene regulation; Genetic Engineering; genome engineering; Genome, Fungal; Genomes; human pathogenic fungi; Humans; Mammalian cells; Mutation; off-target events; Plant cells; Plasmids; Proteins; sgRNA; CRISPR; off-target events; gene regulation; sgRNA; Candida; human pathogenic fungi; double-strand break; CAS9; fungal genetics; genome engineering
• ISSN: 1746-0913; 1746-0921
• DOI: 10.2217/fmb-2019-0183

Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-Cas systems have emerged as a powerful tool for genome manipulation. Class 2 type II CRISPR/ is so far the most studied system and has been implemented in many biological systems such as mammalian cells, plants, fungi and bacteria. Fungi are important causes of human diseases worldwide. Genetic manipulation of pathogenic fungi is critical to develop new therapeutic approaches and novel antifungals. We will review here the progress done with CRISPR/ systems in human pathogenic fungi, with emphasis in and the main modifications that have improved their usefulness in biological research. We finally discuss possible future outcomes and applications to the developed in a near future.