Rate of alteration of hepatic mixed-function oxidase system in rats fed different dietary fats

• Published in: Biochemistry and cell biology Vol. 71; no. 11-12; p. 530
• Main Authors: Ammouche, A, Dinh, L, Youyou, A, Clément, M, Bourre, J M
• Format: Journal Article
• Language: English
• Published: Canada 01.11.1993
• Subjects: Aminopyrine N-Demethylase - analysis; Aniline Hydroxylase - analysis; Animals; Cytochrome P-450 Enzyme System - analysis; Cytochrome Reductases - analysis; Cytochrome-B Reductase; Cytochromes b5 - analysis; Dietary Fats - metabolism; Fatty Acids - analysis; Fish Oils - metabolism; Male; Microsomes, Liver - chemistry; Microsomes, Liver - enzymology; Mixed Function Oxygenases - analysis; NADPH-Ferrihemoprotein Reductase - analysis; Plant Oils - metabolism; Rats; Rats, Sprague-Dawley; Weaning
• ISSN: 0829-8211
• DOI: 10.1139/o93-076

Studies were carried out to evaluate and relate the rate of alteration in mixed-function oxidase system with the changes of the fatty acid composition of rat microsomes induced by different dietary lipids. Male weanling rats were fed from day 21 to 120 with a commercial rat diet or a semisynthetic diet containing no fat or 10% fat consisting of peanut-rapeseed oil, sunflower oil, or salmon oil. In rats fed a fat-free diet, the cytochrome P-450 concentration and aniline hydroxylase, aminopyrine N-demethylase, and NADPH-cytochrome-c reductase activities of liver microsomes at 120 days were, respectively, 26, 16, 10, and 24% lesser than those of rats fed the control diet. However, cytochrome b5 concentration and NADH-cytochrome-b5 reductase activity were, respectively, 33 and 43% higher than those of the control group at the same time. When rats were fed the sunflower oil diet, the cytochrome P-450 concentration and NADH-cytochrome-b5 reductase activity at 120 days were, respectively, 11 and 23% lesser than those of control group. But the cytochrome b5 concentration was 10% higher than that of the control group. In rats fed the fish oil diet, the cytochrome P-450 concentration and NADPH-cytochrome-c reductase, aniline hydroxylase, and aminopyrine N-demethylase activities at 120 days were, respectively, 30, 48, 41, and 31% higher than those of rats fed the control diet. These enzymes were correlated very well (0.84 < r < 0.93), P < 0.05 with dietary sigma polyunsaturated fatty acids (n-3).