Sweet diterpenic glycosides balance of a new cultivar of Stevia rebaudiana (Bert.) Bertoni: Isolation and quantitative distribution by chromatographic, spectroscopic, and electrophoretic methods
A dichloromethane fraction and a ultrafiltered water extract of leaves of a new cultivar of Stevia rebaudiana were submitted to separated downstreaming fractionation in silica gel columns. Non-glycosylated and glycosylated pairs for stigmasterol and β-sistosterol were obtained from the former. Stevi...
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| Published in | Process biochemistry (1991) Vol. 40; no. 11; pp. 3587 - 3594 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
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Elsevier Ltd
01.11.2005
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| Abstract | A dichloromethane fraction and a ultrafiltered water extract of leaves of a new cultivar of
Stevia rebaudiana were submitted to separated downstreaming fractionation in silica gel columns. Non-glycosylated and glycosylated pairs for stigmasterol and β-sistosterol were obtained from the former. Stevioside and rebaudioside-A were resolved from the second hydrophilic extract. The structures of these natural compounds were then confirmed through gas chromatography coupled to mass spectrometry and hydrogen/carbon nuclear magnetic resonance. The main sweetener glycoside pair, following scaled-up water extraction, and sugar derivatives downstreaming by membrane filtration and ionic resin exchange, was analyzed by thin layer densitometered chromatography, high-pressure liquid chromatography, and capillary electrophoresis. Thus, an unusual sweetener ratio on the behalf of rebaudioside-A, a glycoside almost devoid of bitter after-taste, was found for a new
Stevia cultivar (UEM-320) as compared to other native cultivars, where the bitterer stevioside was the major diterpenic glycoside. |
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| AbstractList | A dichloromethane fraction and a ultrafiltered water extract of leaves of a new cultivar of
Stevia rebaudiana were submitted to separated downstreaming fractionation in silica gel columns. Non-glycosylated and glycosylated pairs for stigmasterol and β-sistosterol were obtained from the former. Stevioside and rebaudioside-A were resolved from the second hydrophilic extract. The structures of these natural compounds were then confirmed through gas chromatography coupled to mass spectrometry and hydrogen/carbon nuclear magnetic resonance. The main sweetener glycoside pair, following scaled-up water extraction, and sugar derivatives downstreaming by membrane filtration and ionic resin exchange, was analyzed by thin layer densitometered chromatography, high-pressure liquid chromatography, and capillary electrophoresis. Thus, an unusual sweetener ratio on the behalf of rebaudioside-A, a glycoside almost devoid of bitter after-taste, was found for a new
Stevia cultivar (UEM-320) as compared to other native cultivars, where the bitterer stevioside was the major diterpenic glycoside. A dichloromethane fraction and a ultrafiltered water extract of leaves of a new cultivar of Stevia rebaudiana were submitted to separated downstreaming fractionation in silica gel columns. Non-glycosylated and glycosylated pairs for stigmasterol and 13-sistosterol were obtained from the former. Stevioside and rebaudioside-A were resolved from the second hydrophilic extract. The structures of these natural compounds were then confirmed through gas chromatography coupled to mass spectrometry and hydrogen/carbon nuclear magnetic resonance. The main sweetener glycoside pair, following scaled-up water extraction, and sugar derivatives downstreaming by membrane filtration and ionic resin exchange, was analyzed by thin layer densitometered chromatography, high-pressure liquid chromatography, and capillary electrophoresis. Thus, an unusual sweetener ratio on the behalf of rebaudioside-A, a glycoside almost devoid of bitter after-taste, was found for a new Stevia cultivar (UEM-320) as compared to other native cultivars, where the bitterer stevioside was the major diterpenic glycoside. |
| Author | Adelmann, Juliana da Costa, Cecília E.M. da Costa, Silvio Cláudio Dacome, Antonio S. da Silva, Cleuza C. Fontana, José D. |
| Author_xml | – sequence: 1 givenname: Antonio S. surname: Dacome fullname: Dacome, Antonio S. organization: Departamento de Química, Universidade Estadual de Maringá, Av. Colombo 5790, CEP 87020-900, Maringá, PR, Brazil – sequence: 2 givenname: Cleuza C. surname: da Silva fullname: da Silva, Cleuza C. organization: Departamento de Química, Universidade Estadual de Maringá, Av. Colombo 5790, CEP 87020-900, Maringá, PR, Brazil – sequence: 3 givenname: Cecília E.M. surname: da Costa fullname: da Costa, Cecília E.M. organization: Departamento de Bioquímica, Núcleo de Estudos em Produtos Naturais, Universidade Estadual de Maringá, Av. Colombo 5790, CEP 87020-900, Maringá, PR, Brazil – sequence: 4 givenname: José D. surname: Fontana fullname: Fontana, José D. organization: LQBB-Biomass Chemo/Biotechnology Laboratory, Department of Pharmacy, Federal University of Parana, Av. Lothario Meissner 3400, CEP 80210-170, Curitiba, PR, Brazil – sequence: 5 givenname: Juliana surname: Adelmann fullname: Adelmann, Juliana organization: LQBB-Biomass Chemo/Biotechnology Laboratory, Department of Pharmacy, Federal University of Parana, Av. Lothario Meissner 3400, CEP 80210-170, Curitiba, PR, Brazil – sequence: 6 givenname: Silvio Cláudio surname: da Costa fullname: da Costa, Silvio Cláudio email: sccosta@uem.br organization: Departamento de Bioquímica, Núcleo de Estudos em Produtos Naturais, Universidade Estadual de Maringá, Av. Colombo 5790, CEP 87020-900, Maringá, PR, Brazil |
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| Cites_doi | 10.4141/P97-114 10.1016/S0031-9422(00)88792-0 10.1080/10826079508010001 10.1248/cpb.25.3437 10.1271/bbb1961.40.2103 10.1021/jf00116a056 10.1016/S0040-4039(00)77990-1 10.1271/bbb1961.54.3137 10.1016/S0031-9422(00)84384-8 10.1248/cpb.50.1007 10.1016/S0031-9422(01)00416-2 10.1002/elps.1150170213 10.1248/cpb.25.844 10.1039/np9931000301 10.1016/0031-9422(90)83073-A |
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Sterol glucosides from Prunella vulgaris publication-title: Phytochemistry doi: 10.1016/0031-9422(90)83073-A contributor: fullname: Kojima |
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Stevia rebaudiana were submitted to separated downstreaming... A dichloromethane fraction and a ultrafiltered water extract of leaves of a new cultivar of Stevia rebaudiana were submitted to separated downstreaming... |
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| Title | Sweet diterpenic glycosides balance of a new cultivar of Stevia rebaudiana (Bert.) Bertoni: Isolation and quantitative distribution by chromatographic, spectroscopic, and electrophoretic methods |
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