Sweet diterpenic glycosides balance of a new cultivar of Stevia rebaudiana (Bert.) Bertoni: Isolation and quantitative distribution by chromatographic, spectroscopic, and electrophoretic methods

• Published in: Process biochemistry (1991) Vol. 40; no. 11; pp. 3587 - 3594
• Main Authors: Dacome, Antonio S., da Silva, Cleuza C., da Costa, Cecília E.M., Fontana, José D., Adelmann, Juliana, da Costa, Silvio Cláudio
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.11.2005
• Subjects: HPLC; Rebaudioside-A; Stevia rebaudiana; Stevioside; Sweeteners; TLC; CE; Rebaudioside-A; Stevioside; Stevia rebaudiana; TLC; Sweeteners; HPLC
• ISSN: 1359-5113; 1873-3298
• DOI: 10.1016/j.procbio.2005.03.035

A dichloromethane fraction and a ultrafiltered water extract of leaves of a new cultivar of Stevia rebaudiana were submitted to separated downstreaming fractionation in silica gel columns. Non-glycosylated and glycosylated pairs for stigmasterol and β-sistosterol were obtained from the former. Stevioside and rebaudioside-A were resolved from the second hydrophilic extract. The structures of these natural compounds were then confirmed through gas chromatography coupled to mass spectrometry and hydrogen/carbon nuclear magnetic resonance. The main sweetener glycoside pair, following scaled-up water extraction, and sugar derivatives downstreaming by membrane filtration and ionic resin exchange, was analyzed by thin layer densitometered chromatography, high-pressure liquid chromatography, and capillary electrophoresis. Thus, an unusual sweetener ratio on the behalf of rebaudioside-A, a glycoside almost devoid of bitter after-taste, was found for a new Stevia cultivar (UEM-320) as compared to other native cultivars, where the bitterer stevioside was the major diterpenic glycoside.