Striatal dopamine in the response of brain and liver unsaturated and saturated free fatty acids following administration of C75 in CD-1 mice

• Published in: Neuroscience letters Vol. 469; no. 3; pp. 324 - 327
• Main Authors: Binienda, Z.K., Ross, I.A., Gough, B., Riccio, T., Kim, C.S., Ali, S.F.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 29.01.2010; Elsevier
• Subjects: 4-Butyrolactone - administration & dosage; 4-Butyrolactone - analogs & derivatives; 4-Butyrolactone - pharmacology; Animals; Anti-Obesity Agents - administration & dosage; Anti-Obesity Agents - pharmacology; Biogenic Monoamines - metabolism; Biological and medical sciences; Brain; Brain - drug effects; Brain - metabolism; C75; Cerulenin - analogs & derivatives; Corpus Striatum - drug effects; Corpus Striatum - metabolism; Dopamine; Dopamine - metabolism; Dose-Response Relationship, Drug; Eating - drug effects; Fatty Acids, Monounsaturated - metabolism; Fatty Acids, Nonesterified - metabolism; Fatty Acids, Unsaturated - metabolism; Free fatty acids; Fundamental and applied biological sciences. Psychology; Liver; Liver - metabolism; Male; Mice; Mice, Inbred Strains; Neurotransmitter Agents - metabolism; Serotonin; Serotonin - metabolism; Time Factors; Vertebrates: nervous system and sense organs; C75; Brain; Dopamine; Serotonin; Liver; Free fatty acids; Digestive system; Rodentia; Central nervous system; Lipids; Basal ganglion; Corpus striatum; Free fatty acid; Catecholamine; Encephalon; Vertebrata; Mammalia; Mouse; Saturated fatty acid; Animal; Neurotransmitter
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/j.neulet.2009.12.019

In order to clarify the mechanism of action of cerulenin analog, C75, known to suppress feeding behavior, food intake was measured in adult CD-1 male mice n = 5 per group, treated i.p. with 10 and 20 mg/kg of C75. Animals in both treatment groups had significantly lower 24 h food consumption rate relative to the control group injected with vehicle. Striatal monoamine neurotransmitters and striatal as well as liver long chain free fatty acids concentrations were subsequently evaluated in another group treated i.p. with 20 mg/kg C75. Acute exposure to C75 at 20 mg/kg led to approximately 50% increase in the striatal dopamine levels and a decrease in dopamine turnover for up to 24 h following the injection. The concentration of serotonin remained unchanged. Concentration of saturated fatty acids in the liver and striatum did not change, while striatal unsaturated myristoleic acid (cis-9-tetradecenoic acid) levels were significantly higher as early as 2 h post-injection and remained elevated at 24 h post-injection. These preliminary data suggest a central regulatory role of unsaturated fatty acids under dopaminergic control in the C75-induced anorexia. Pharmacological alterations in fatty acid metabolism may prove beneficial in the treatment of obesity.