Forward RNAi screens in human stem cells

Identifying the genes and pathways that regulate self-renewal and differentiation in somatic stem cells is a central goal in stem cell and cancer biology. Here, we describe a method for RNAi-based screens in primary human hematopoietic stem and progenitor cells. These cells are suitable targets for...

Full description

Saved in:
Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 650; p. 29
Main Authors Karlsson, Christine, Larsson, Jonas, Baudet, Aurélie
Format Journal Article
LanguageEnglish
Published United States 2010
Subjects
Cells, Cultured
Hematopoietic Stem Cells - cytology
Hematopoietic Stem Cells - metabolism
Humans
Models, Biological
RNA Interference - physiology
Stem Cells - cytology
Stem Cells - metabolism
Online AccessGet more information

Cover

More Information
Summary:Identifying the genes and pathways that regulate self-renewal and differentiation in somatic stem cells is a central goal in stem cell and cancer biology. Here, we describe a method for RNAi-based screens in primary human hematopoietic stem and progenitor cells. These cells are suitable targets for complex, selection-based screens using pooled lentiviral shRNA libraries. The screening approach is a promising new tool to dissect regulatory mechanisms in hematopoietic and somatic stem cells, in general, and may be particularly useful to identify gene targets and modifiers that can be further exploited in strategies for ex vivo stem cell expansion.
ISSN:1940-6029
DOI:10.1007/978-1-60761-769-3_3