Phytochemical analysis and evaluation of the antioxidant and antiproliferative effects of Tucumã oil nanocapsules in breast adenocarcinoma cells (MCF-7)

• Published in: Natural product research Vol. 35; no. 12; pp. 2060 - 2065
• Main Authors: Nascimento, Kátia, Copetti, Priscila Marquezan, Fernandes, Andressa, Klein, Bruna, Fogaça, Aline, Zepka, Leila Queiroz, Wagner, Roger, Ourique, Aline Ferreira, Sagrillo, Michele Rorato, da Silva, José Edson Paz
• Format: Journal Article
• Language: English
• Published: Abingdon Taylor & Francis 01.06.2021; Taylor & Francis Ltd
• Subjects: Adenocarcinoma; Anticancer properties; Antioxidants; Antiproliferatives; Antitumor activity; Assaying; Breast; breast cancer; Carotene; Cytotoxicity; nanocapsules; Nitric oxide; Oil; Phytosterols; Squalene; Subsidies; Toxicity; tucumã; β-Carotene
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2019.1648460

In this work was to develop an inedited nanocapsule with tucumã oil (Astrocaryum vulgare). The oil presents of phytosterols (squalene and β-sitosterol), all-trans-beta-carotene, acids oleic and palmitic. Antioxidant activity showed a good performance in DPPH and ABTS assays. The nanocapsules were prepared and demonstrated in their characterization particle size (206 ± 0.69 nm). The cytogenotoxicity evaluation was performed using the MTT, dichlorofluorescein, nitric oxide and dsDNA PicoGreen® assays. Antitumor efficacy assays in MCF-7 cells demonstrated that free oil and tucumã nanocapsules had IC 50 of 130 and 50 μg/mL, respectively. Thus, previous studies of toxicity are relevant, as they generate future subsidies, aiming at the potential application of nanostructures and in addition, the promising effect of NCs of tucumã oil on the antiproliferative effect in breast adenocarcinoma cells was evidenced.