Counting single molecules in sub-nanolitre droplets

We demonstrate single biomolecule detection and quantification within sub-nanolitre droplets through application of Cylindrical Illumination Confocal Spectroscopy (CICS) and droplet confinement within a retractable microfluidic constriction.

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Bibliographic Details
Published inLab on a chip Vol. 10; no. 2; pp. 161 - 164
Main Authors Rane, T D, Puleo, C M, Liu, K J, Zhang, Y, Lee, A P, Wang, T H
Format Journal Article
LanguageEnglish
Published England 01.01.2010
Subjects
DNA - analysis
DNA - ultrastructure
Equipment Design
Equipment Failure Analysis
Microfluidic Analytical Techniques - instrumentation
Microscopy, Confocal - instrumentation
Microscopy, Confocal - methods
Nanostructures - analysis
Nanostructures - ultrastructure
Nanotechnology - instrumentation
Particle Size
Reproducibility of Results
Sensitivity and Specificity
Solutions - analysis
Spectrometry, Fluorescence - instrumentation
Spectrometry, Fluorescence - methods
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Summary:We demonstrate single biomolecule detection and quantification within sub-nanolitre droplets through application of Cylindrical Illumination Confocal Spectroscopy (CICS) and droplet confinement within a retractable microfluidic constriction.
Bibliography:TDR and CMP contributed equally to this work.
ISSN:1473-0197
1473-0189
DOI:10.1039/b917503b