Improvement effect of compound Ento-PB on oxazolone-induced ulcerative colitis in rats

• Published in: Acta Cirúrgica Brasileira Vol. 39; p. e395524
• Main Authors: Fan, Zhi, Chen, Jinhu, Wei, Jia, Yang, ZhiBin, Xiao, Huai, Liu, Heng
• Format: Journal Article
• Language: English
• Published: Brazil Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 01.01.2024
• Subjects: Animals; Anti-inflammatory Agents; Colitis, Ulcerative; Colitis, Ulcerative - chemically induced; Colitis, Ulcerative - drug therapy; Colitis, Ulcerative - pathology; Colon - drug effects; Colon - metabolism; Colon - pathology; Cytokines - metabolism; Disease Models, Animal; Drugs, Chinese Herbal - pharmacology; Drugs, Chinese Herbal - therapeutic use; Enzyme-Linked Immunosorbent Assay; Epidermal Growth Factor - analysis; Interleukin-13 - analysis; Intestinal Mucosa - drug effects; Intestinal Mucosa - metabolism; Intestinal Mucosa - pathology; Male; Nitric Oxide Synthase Type II - analysis; Nitric Oxide Synthase Type II - metabolism; Original; Oxazolone; Peroxidase - analysis; Peroxidase - metabolism; Rats; Rats, Sprague-Dawley; Reproducibility of Results; Treatment Outcome; Tumor Necrosis Factor-alpha - analysis; Tumor Necrosis Factor-alpha - metabolism
• ISSN: 0102-8650; 1678-2674
• DOI: 10.1590/acb395524

To investigate the impact of the Chinese medicine compound Ento-PB on oxazolone (OXZ)-induced ulcerative colitis (UC) in rats. UC rats induced by OXZ were treated with Ento-PB. The damage to the colon was assessed using several measures, including the disease activity index (DAI), colon length, colon weight/length ratio, colonic mucosal damage index, and histological score. The levels of interleukin-4 (IL-4), interleukin-10 (IL-10), interleukin-13 (IL-13), epidermal growth factor (EGF), inducible nitric oxide synthase, and total nitric oxide synthase (tNOS) in rat serum, as well as the levels of tumor necrosis factor-α (TNF-α) and myeloperoxidase (MPO) in rat colon tissue, were determined using enzyme-linked immunosorbent assay and conventional kits. After being treated with Ento-PB, the DAI score and macroscopic lesion score of OXZ-induced UC rats were significantly reduced. Ento-PB prevented the shortening of rat colons, reduced the ratio of colon weight to length, and improved colon tissue lesions. Meanwhile, Ento-PB could significantly inhibit the activities of proinflammatory cytokines TNF-α, IL-13, and MPO, as well as tNOS and iNOS, while upregulating the expression of anti-inflammatory cytokines IL-4 and IL-10. Moreover, a significant increase in the expression level of EGF was observed in UC rats treated with Ento-PB, indicating that Ento-PB could enhance the repair of damaged intestinal epithelial tissue. Ento-PB demonstrates significant anti-UC activities in OXZ-induced UC rats by regulating the expression levels of inflammatory factors and promoting the repair of colon tissue. This study provides scientific evidence to support the further development of Ento-PB.