Progesterone Synthesis and Fine Structure of Dissociated Monkey (Macaca mulatta) Luteal Cells Maintained in Culture
The process of functional regression of the primate corpus luteum in the menstrual cycle is not well understood, yet it remains an important determinant in fertility management, including contraceptive actions. Accordingly, the feasibility of maintaining rhesus monkey luteal cells in culture for lon...
Saved in:
Published in | Biology of reproduction Vol. 20; no. 4; pp. 779 - 792 |
---|---|
Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
Society for the Study of Reproduction
01.05.1979
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | The process of functional regression of the primate corpus luteum in the menstrual cycle is not
well understood, yet it remains an important determinant in fertility management, including contraceptive actions. Accordingly,
the feasibility of maintaining rhesus monkey luteal cells in culture for
long term studies was explored. When midluteal phase corpus luteum (CL) was dispersed with
collagenase in Hamâs F10 (F10) medium, 60% of the initial 5 x 10 4 cells plated attached. Unattached cells were removed in media changes on Day 2 and 4. Trypan blue exclusion showed that
60-80% of unattached cells were dead or dying. At 10 days of culture, the number of cells per
dish in F10 was comparable to the number of cells when D-valine was substituted for L-valine
(D-val-F10) and when 100 ng hCG/ml was added to F10. The number of cells was slightly higher
when cultured in Hankâs M199 (M199) medium. Progesterone (P) production in F10 medium
declined markedly between Day 2 and Day 6, but remained detectable until Day 10. Although
hCG increased P production (P<0.01) throughout the 10 days of culture, it failed to prevent the
pattern of diminished P production. Cells cultured in F10 for 6 days before exposure to hCG
increased P production (P<0.01) during the next 2 days. P production in D-val-F10 was similar to
F10 medium; however, in M199 medium it was 2-fold greater (P<0.01). Cytologically, a large and
a smaller luteal cell population was identified after dissociation. The large cells had agranular ER
(AER) distributed peripherally, whereas the smaller cells had more granular ER (GER) and Golgi.
Except for the cultures containing hCG, cultured cells appeared similar. Regardless of composition
of culture medium, 3-4% of the cells in culture were large. In these large cells, the zone of peripheral AER was absent and
mitochondria became elongated with increased age in culture. The smaller
luteal cells were the predominant type throughout the culture period. The smaller luteal cells in
culture in F10, D-val-F10 and M199 had diminished quantities of GER and increased number of
polysomes with advancing age in culture. Smaller luteal cells, maintained in the presence of hCG
for 4 days of longer, had more AER, large ovoid nuclei and thread-like branching mitochondria.
We present evidence that these monkey luteal cells can be maintained for at least 10 days in the
condition described here. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0006-3363 1529-7268 |
DOI: | 10.1095/biolreprod20.4.779 |