The E-Box of the Human Glycophorin B Promoter Is Involved in the Erythroid-Specific Expression of the GPB Gene

Previous studies performed on the glycophorin B (GPB) expression demonstrated that this gene is expressed in erythroid cells only and that the ubiquitous factor Ku70 is involved in the process. Here, we investigated the contribution of the −70 E-box sequence toward the GPB promoter expression. We fo...

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Published inBiochemical and biophysical research communications Vol. 265; no. 1; pp. 170 - 176
Main Authors Camara-Clayette, Valérie, Rahuel, Cécile, Bertrand, Olivier, Cartron, Jean-Pierre
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 11.11.1999
Subjects
Antigens, Nuclear
Base Sequence
blood group proteins
Chloramphenicol O-Acetyltransferase - genetics
DNA Helicases
DNA-Binding Proteins - metabolism
erythroid-specific expression
Gene Expression Regulation
Genes, Reporter
Glycophorin - genetics
HeLa Cells
Humans
K562 Cells
Ku Autoantigen
Mutagenesis, Site-Directed
Nuclear Proteins - metabolism
Oligodeoxyribonucleotides
Point Mutation
Promoter Regions, Genetic
Recombinant Fusion Proteins - biosynthesis
repressors
transcription factors
Transcription Factors - metabolism
Transfection
Upstream Stimulatory Factors
repressors
blood group proteins
erythroid-specific expression
transcription factors
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Summary:Previous studies performed on the glycophorin B (GPB) expression demonstrated that this gene is expressed in erythroid cells only and that the ubiquitous factor Ku70 is involved in the process. Here, we investigated the contribution of the −70 E-box sequence toward the GPB promoter expression. We found that the E-box bound two factors, the USF1/USF2 protein and an unidentified ubiquitous protein which was named factor U. Site-directed mutagenesis performed on the −70 E-box showed that the USF factor had an activating effect in CAT assays. Conversely, mutation of the −70 E-box that impaired the binding of factor U led to a positive CAT activity in nonerythroid cells and thus to the loss of the erythroid-specific expression of the GPB gene. This indicates that, in addition to the Ku70 factor, the extinction of the GPB promoter expression in nonerythroid cells depends also on the repressing effect of the factor U.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1999.1634