Novel Fibroblast Growth Factor 2 Transcripts Are Expressed in Mouse Embryos

We have discovered two new exons in the mouse fibroblast growth factor 2 (FGF-2 or bFGF) gene that can be alternatively spliced to the second coding exon of the gene. The newly identified exons 1b and 1c are located at, respectively, approximately 19 and 32 kb downstream of the canonical exon 1a. Us...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 287; no. 4; pp. 900 - 909
Main Authors Dirks, Ron P.H., Potter, Sarah J., Griep, Anne E.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 05.10.2001
Subjects
Alternative Splicing - genetics
alternative transcript
Amino Acid Sequence
Animals
Base Sequence
Blotting, Northern
chick altFGF2
Chromosome Mapping
Embryo, Mammalian - metabolism
Exons - genetics
Eye - metabolism
fibroblast growth factor 2
Fibroblast Growth Factor 2 - chemistry
Fibroblast Growth Factor 2 - genetics
Fibroblast Growth Factor 2 - metabolism
limb development
Mice
Molecular Sequence Data
mouse development
ocular development
placenta
RNA - genetics
RNA - metabolism
Sequence Alignment
Tissue Distribution
limb development
chick altFGF2
fibroblast growth factor 2
placenta
ocular development
mouse development
alternative transcript
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Summary:We have discovered two new exons in the mouse fibroblast growth factor 2 (FGF-2 or bFGF) gene that can be alternatively spliced to the second coding exon of the gene. The newly identified exons 1b and 1c are located at, respectively, approximately 19 and 32 kb downstream of the canonical exon 1a. Using RT-PCR analysis, mRNAs containing exon 1c and canonical exons 2 and 3 were identified in embryonic limb, placenta, face, carcass and ocular tissues. A 3.7-kb transcript present in placenta and embryonic limb hybridizes with an exon 1c-derived probe in Northern blot analysis. Alternative splicing of exon 1c to exon 2 creates a transcript for which the predicted alternative FGF-2 (altFGF-2) polypeptide contains a novel N-terminal domain. Our data indicate that in mouse embryos multiple novel mRNA variants are transcribed from the FGF-2 locus using alternative splicing. These data suggest that proteins arising from these alternative transcripts may play a role in mouse embryogenesis.
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ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2001.5683