Process for the production of tilapia retorted skin gelatin hydrolysates with optimized antioxidative properties

• Published in: Process biochemistry (1991) Vol. 44; no. 10; pp. 1152 - 1157
• Main Authors: Yang, Jing-Iong, Liang, Wen-Sing, Chow, Chau-Jen, Siebert, Karl J.
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.10.2009
• Subjects: Antioxidative activity; Hydrolysate; Optimization; Skin gelatin; Thermal hydrolysis; Tilapia; Tilapia; Antioxidative activity; Skin gelatin; Thermal hydrolysis; Hydrolysate; Optimization
• ISSN: 1359-5113; 1873-3298
• DOI: 10.1016/j.procbio.2009.06.013

Thermally hydrolyzed tilapia skin gelatin demonstrated noticeable free-radical scavenging and inhibition of lipid peroxidation. Five factors in production of retorted skin gelatin hydrolysate (RSGH) were screened using a fractional factorial design to identify critical factors. Phosphoric acid concentration, water/skin ratio, and retorting time had significant effects on α,α-diphenyl-β-picrylhydrazyl (DPPH) scavenging by RSGHs. A face-centered, central composite design in these three factors was used to collect data that resulted in strong response surface models of DPPH scavenging ( R 2 = 0.977) and inhibition of lipid peroxidation ( R 2 = 0.967). The most effective condition resulted in 80.3% DPPH scavenging and 75.0% inhibition of lipid peroxidation. The models were used to predict maxima for the two properties. These were 79.4% for DPPH scavenging activity and 77.3% for lipid peroxidation inhibition. Antioxidative tilapia RSGH has potential as a natural antioxidant because a large amount of low-priced skin by-products can be obtained from the tilapia filleting industry.