Chlorophyll a fluorescence and growth of Neoregelia concentrica (Bromeliaceae) during acclimatization in response to light levels

• Published in: In vitro cellular & developmental biology. Plant Vol. 51; no. 4; pp. 471 - 481
• Main Authors: Martins, João Paulo Rodrigues, Schimildt, Edilson Romais, Alexandre, Rodrigo Sobreira, Falqueto, Antelmo Ralph, Otoni, Wagner Campos
• Format: Journal Article
• Language: English
• Published: New York Springer 01.08.2015; Springer US; Springer Nature B.V
• Subjects: acclimation; Acclimatization; Acids; Biomedical and Life Sciences; Cell Biology; Chlorophyll; Developmental Biology; Flowers & plants; Fluorescence; Growth rate; Life Sciences; Light; light intensity; mortality; Neoregelia; photoinhibition; Physiology; Plant Breeding/Biotechnology; plant cultural practices; Plant Genetics and Genomics; Plant Sciences; Plant species; PLANT TISSUE CULTURE; Seeds; solar radiation; Studies; Sucrose; Sunlight; tissue culture; trays; vermiculite; Photoinhibition; Tissue culture; Acclimatization; JIP test; Bromeliad
• ISSN: 1054-5476; 1475-2689
• DOI: 10.1007/s11627-015-9711-z

Tissue culturing is widely used for the clonal propagation of several plant species. However, high mortality rates may occur during the acclimatization phase due to the sudden, dramatic changes in light levels. This study evaluated the effect of light level during the acclimatization of Neoregelia concentrica (Vellozo) LB Smith using chlorophyll a fluorescence and growth analysis. N. concentrica plants were multiplied and rooted in vitro on Murashige and Skoog medium and then transferred to plastic trays with individual cells containing vermiculite. The treatments consisted of the ex vitro cultivation of plants under light levels of 30, 50, 70, and full sunlight (100% light) for 28 d. Chlorophyll a fluorescence measurements were taken at 0 (in vitro), 7, 14, 21, and 28 d of acclimatization, and growth trait analyses were made at 28 d. The high irradiance under full sunlight (100% light) induced photoinhibition and reduced the growth rate. Plants grown under partial light (30, 50, and 70%) were less inhibited. A 50% reduction in light level during N. concentrica acclimatization produced rapid plant recovery and higher growth rates than full sunlight.