Improved avidin-biotin-peroxidase complex (ABC) staining
A considerable intensification of the avidin-biotin-peroxidase complex staining system (ABC) was obtained by sequentially overlaying the sections to be immunostained with an avidin-rich and a biotin-rich complex. Each sequential addition contributed to the deposition of horseradish peroxidase on the...
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Published in | The Histochemical journal Vol. 20; no. 2; p. 75 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
01.02.1988
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Subjects | |
Online Access | Get more information |
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Summary: | A considerable intensification of the avidin-biotin-peroxidase complex staining system (ABC) was obtained by sequentially overlaying the sections to be immunostained with an avidin-rich and a biotin-rich complex. Each sequential addition contributed to the deposition of horseradish peroxidase on the immunostained site and allowed the subsequent binding of a complementary complex. With this technique a higher dilution of the antisera could be used and minute amounts of antigen masked by the fixative could be demonstrated on paraffin sections. |
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ISSN: | 0018-2214 |
DOI: | 10.1007/bf01746607 |