Improved avidin-biotin-peroxidase complex (ABC) staining

A considerable intensification of the avidin-biotin-peroxidase complex staining system (ABC) was obtained by sequentially overlaying the sections to be immunostained with an avidin-rich and a biotin-rich complex. Each sequential addition contributed to the deposition of horseradish peroxidase on the...

Full description

Saved in:
Bibliographic Details
Published inThe Histochemical journal Vol. 20; no. 2; p. 75
Main Authors Cattoretti, G, Berti, E, Schiró, R, D'Amato, L, Valeggio, C, Rilke, F
Format Journal Article
LanguageEnglish
Published Netherlands 01.02.1988
Subjects
Avidin
Biotin
Histocytochemistry
Horseradish Peroxidase
Humans
Peroxidases
Staining and Labeling - methods
Online AccessGet more information

Cover

More Information
Summary:A considerable intensification of the avidin-biotin-peroxidase complex staining system (ABC) was obtained by sequentially overlaying the sections to be immunostained with an avidin-rich and a biotin-rich complex. Each sequential addition contributed to the deposition of horseradish peroxidase on the immunostained site and allowed the subsequent binding of a complementary complex. With this technique a higher dilution of the antisera could be used and minute amounts of antigen masked by the fixative could be demonstrated on paraffin sections.
ISSN:0018-2214
DOI:10.1007/bf01746607