Enhancement of Endothelial Cell E-Selectin Expression by Sera from Patients with Active Behçet's Disease: Moderate Correlation with Anti-endothelial Cell Antibodies and Serum Myeloperoxidase Levels

• Published in: Clinical immunology (Orlando, Fla.) Vol. 91; no. 3; pp. 330 - 337
• Main Authors: Triolo, Giovanni, Accardo-Palumbo, Antonina, Triolo, Giuseppa, Carbone, Maria Carmela, Ferrante, Angelo, Giardina, Ennio
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.1999
• Subjects: adhesion molecules; Adult; Aged; anti-endothelial cell antibodies; Antibodies, Antineutrophil Cytoplasmic - blood; Autoantibodies - blood; Behcet Syndrome - blood; Behcet Syndrome - enzymology; Behcet Syndrome - immunology; Behçet's disease; Cells, Cultured; Cytokines - blood; E-Selectin - blood; E-Selectin - metabolism; Endothelium, Vascular - immunology; Female; Humans; Immunoglobulin G - blood; In Vitro Techniques; Male; Middle Aged; myeloperoxidase; Neutrophils - enzymology; Peroxidase - blood; Peroxidase - immunology; anti-endothelial cell antibodies; Behçet's disease; myeloperoxidase; adhesion molecules
• ISSN: 1521-6616; 1521-7035
• DOI: 10.1006/clim.1999.4687

We studied thein vitroE-selectin expression of endothelial cells treated with sera from patients with Behçet's disease (BD) and factors (anti-endothelial cell antibodies, anti-neutrophil cytoplasmic antibodies, cytokines, and myeloperoxidase (MPO)) that may contribute to adhesion molecule expression. A total of 21 patients with BD and 27 healthy controls were studied.In vitroE-selectin endothelial cell expression was investigated by ELISA after HUVEC incubation with sera or purified IgG from patients with BD and controls. Increased E-selectin expression was observed when endothelial cells were incubated with sera from patients with active disease or from patients with circulating anti-endothelial cell antibodies and high levels of plasma myeloperoxidase. Abnormalities of endothelial cell function have been suggested to play a role in the occurrence of vascular damage in BD. Our findings suggest that anti-endothelial cell antibodies and neutrophil hyperactivity, as inferred from the high plasma MPO levels in patients with active disease, may explain endothelial cell activation and neutrophil accumulation in vascular lesions.