attempt to correlate fat and protein content of biological samples with residual carbon after microwave-assisted digestion

• Published in: Analytical and bioanalytical chemistry Vol. 371; no. 4; pp. 536 - 540
• Main Authors: CARRILHO, Elma Neide V. M, NOGUEIRA, Ana Rita A, NOBREGA, Joaquim A, DE SOUZA, Gilberto B, CRUZ, Geraldo M
• Format: Journal Article
• Language: English
• Published: Berlin Springer-Verlag 01.10.2001; Springer
• Subjects: Analysis; Animals; Biological and medical sciences; Biological specimens; Carbon - analysis; Cattle; Ether - chemistry; Fat cells; Fats - analysis; Food industries; Fundamental and applied biological sciences. Psychology; General aspects; Methods of analysis, processing and quality control, regulation, standards; Microwaves; Proteins; Proteins - analysis; Optical spectrometry; Correlation; Chemical analysis; Bovine; Offals; Fat content; Inductive coupling plasma spectrometry; Vertebrata; Emission spectrometry; Mammalia; Carbon content; Microwave oven; Acid digestion; Artiodactyla; Beef; Animal waste; Ungulata; Protein content
• ISSN: 0937-0633; 1618-2642; 1432-1130; 1618-2650
• DOI: 10.1007/s002160101029

The residual carbon content of a variety of bovine-derived samples and forage was determined by inductively coupled plasma optical emission spectrometry with radial view configuration (ICP–OES) after microwave-assisted digestion under high pressure in a closed vessel. The original carbon concentration in the samples was determined by elemental analysis. The highest amount of original carbon content (64%) was found in viscera. After digestion, up to 75% of it was destroyed. Viscera presented the highest ether extract and blood exhibited a high crude protein content of up to 99%. The efficiency in destroying the organic matter in biological materials seemed to be related to their fat content and showed no significant difficulty for protein-rich samples. The correlation coefficient between the fat content of the samples and the residual carbon after acid decomposition was 0.9173 indicating a fair fit. However, no correlation was observed between % RC and the protein content.